Identification of mosquito bloodmeals using mitochondrial cytochrome oxidase subunit I and cytochrome b gene sequences
Identification of mosquito bloodmeals using mitochondrial cytochrome oxidase subunit I and cytochrome b gene sequences
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DOI：
10.1111/j.1365-2915.2008.00760.x
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发表时间：
2008-12-01
影响因子：
1.9
通讯作者：
Judd, D. D.
中科院分区：
文献类型：
--
作者：
Townzen, J. S.;Brower, A. V. Z.;Judd, D. D.
设计引物并建立了从蚊（双翅目：蚊科）血中选择性扩增脊椎动物线粒体细胞色素氧化酶亚基1（COI）和细胞色素B（Cyt B）mtDNA的方法。该方案使用两对巢式COI引物和一对Cyt B引物来扩增DNA的短片段。然后将所得序列与GenBank中的序列进行比较，使用BLAST功能，用于推定的宿主鉴定。我们从美国俄勒冈州的162只野外捕获的吸血蚊子中扩增了88%的脊椎动物DNA，GenBank BLAST检索鉴定了98%的扩增序列，包括一种两栖动物，七种哺乳动物和14种鸟类。标准和注意事项，推定鉴定血粉进行了讨论。
Primer pairs were designed and protocols developed to selectively amplify segments of vertebrate mitochondrial cytochrome oxidase subunit 1 (COI) and cytochrome b (Cyt b) mtDNA from the bloodmeals of mosquitoes (Diptera: Culicidae). The protocols use two pairs of nested COI primers and one pair of Cyt b primers to amplify short segments of DNA. Resultant sequences are then compared with sequences in GenBank, using the BLAST function, for putative host identification. Vertebrate DNA was amplified from 88% of our sample of 162 wild-caught, blood-fed mosquitoes from Oregon, U.S.A. and GenBank BLAST searches putatively identified 98% of the amplified sequences, including one amphibian, seven mammalian and 14 avian species. Criteria and caveats for putative identification of bloodmeals are discussed.