CO2 evolution and denaturing gradient gel electrophoresis profiles of bacterial communities in soil following addition of low molecular weight substrates to simulate root exudation
CO2 evolution and denaturing gradient gel electrophoresis profiles of bacterial communities in soil following addition of low molecular weight substrates to simulate root exudation
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DOI：
10.1016/s0038-0717(03)00105-6
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发表时间：
2003-06-01
影响因子：
9.7
通讯作者：
Nannipieri, P
中科院分区：
文献类型：
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作者：
Falchini, L;Naumova, N;Nannipieri, P
模拟C-14和C-12-CO2在根面的演变过程中监测C-14-标记的葡萄糖，草酸，或谷氨酸从放置在表面上的桑迪壤土的过滤器到土壤中的扩散。在3 d和7 d后，从4个层（0-2、2-4、4-6和6-14 mm）取样以测定每层中的残留C-14。草酸的矿化模式的特点是滞后期可能是由于存在，在暴露的早期阶段，一些微生物能够矿化这种基板。葡萄糖和谷氨酸表现出积极的启动效应与天然有机物的CO2冲洗。草酸和谷氨酸改变了土壤细菌群落的变性梯度凝胶电泳图谱，在0-2 mm土层出现了少量的额外条带。由于基质在土壤中的扩散，基质在土壤表面的添加形成了梯度。草酸的专一性可能是因为几乎所有的草酸都与CaCO_3反应，并局限于0-2 mm土层中。(C)2002爱思唯尔科技有限公司。保留所有权利。
Simulating the evolution of both C-14 and C-12-CO2 in the rhizoplane was monitored during the diffusion of C-14-labelled glucose, oxalic acid, or glutamic acid into soil from a filter placed on the surface of a sandy loam. After 3 and 7 d, soil was sampled from four layers (0-2, 2-4, 4-6, and 6-14 mm) to determine residual C-14 in each layer. The mineralisation pattern of oxalic acid was characterised by a lag phase probably due to the presence, in the early stages of exposure, of a few microorganisms able to mineralise this substrate. Glucose and glutamic acid showed a positive priming effect with a CO2 flush from native organic matter. Oxalic and glutamic acids changed the denaturing gradient gel electrophoresis profiles of soil bacterial communities with the appearance of a few extra-bands in the 0-2 mm soil layer. The addition of the substrates onto the soil surface formed a gradient due to their diffusion in soil. That of oxalic acid was specific probably because almost all of this compound reacted with CaCO3, and was localised in the 0-2 mm soil layer. (C) 2002 Elsevier Science Ltd. All rights reserved.