The Association of Fried Meat Consumption With the Gut Microbiota and Fecal Metabolites and Its Impact on Glucose Homoeostasis, Intestinal Endotoxin Levels, and Systemic Inflammation: A Randomized Controlled-Feeding Trial
The Association of Fried Meat Consumption With the Gut Microbiota and Fecal Metabolites and Its Impact on Glucose Homoeostasis, Intestinal Endotoxin Levels, and Systemic Inflammation: A Randomized Controlled-Feeding Trial
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DOI：
10.2337/dc21-0099
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发表时间：
2021-09-01
期刊：
影响因子：
16.2
通讯作者：
Han, Tianshu
中科院分区：
文献类型：
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作者：
Gao, Jian;Guo, Xiaoyu;Han, Tianshu
目的本随机对照喂养试验旨在确定油炸肉类摄入对肠道菌群和粪便彗星的影响，以及这些影响是否影响宿主葡萄糖稳态，肠道内毒素水平和全身炎症。研究设计和方法：将117名超重成年人随机分为两组。59名参与者每周提供4次油炸肉，58名参与者限制油炸肉的摄入，同时保持食物组和营养成分不变，持续4周。通过16 S rRNA测序分析肠道菌群。测量口服葡萄糖耐量试验0、30、60和120分钟时的葡萄糖和胰岛素浓度、粪便微生物-宿主共代谢物水平以及肠内毒素和炎症血清生物标志物水平。计算胰岛素曲线下面积（AUC）、胰岛素生成指数（IGI）和肌肉胰岛素抵抗指数（MIRI）。结果食用油炸肉类的受试者IGI值低于对照组，但胰岛素和脂多糖（LPS）、TNF-α、IL-10和IL-1 β水平的MIRI和AUC值高于对照组（P < 0.05）。油炸肉类的摄入降低了微生物群落的丰富度，降低了毛螺菌科和黄曲霉的丰度，同时增加了小杆菌属、多雷亚氏菌属和韦荣氏菌属的丰度（P FDR
OBJECTIVE This randomized controlled-feeding trial aimed to determine the impact of fried meat intake on the gut microbiota and fecal cometabolites and whether such impacts influenced host glucose homoeostasis, intestinal endotoxin levels, and systemic inflammation. RESEARCH DESIGN AND METHODS A total of 117 overweight adults were randomized into two groups. Fifty-nine participants were provided fried meat four times per week, and 58 participants were restricted from fried meat intake, while holding food group and nutrient compositions constant, for 4 weeks. The gut microbiota was analyzed by 16S rRNA sequencing. Glucose and insulin concentrations at 0, 30, 60, and 120 min of an oral glucose tolerance test, fecal microbiota-host cometabolite levels, and intestinal endotoxin and inflammation serum biomarker levels were measured. The area under the curve (AUC) for insulin, insulinogenic index (IGI), and muscle insulin resistance index (MIRI) were calculated. RESULTS The participants who consumed fried meat had lower IGI values than the control subjects, but they had higher MIRI and AUC values of insulin and lipopolysaccharide (LPS), TNF-alpha, IL-10, and IL-1 beta levels (P < 0.05). Fried meat intake lowered microbial community richness and decreased Lachnospiraceae and Flavonifractor abundances while increasing Dialister, Dorea, and Veillonella abundances (P FDR