Distribution and Temporal Regulation of the Immune Response in the Rat Brain to Intracerebroventricular Injection of Interferon-γ
Distribution and Temporal Regulation of the Immune Response in the Rat Brain to Intracerebroventricular Injection of Interferon-γ
复制标题
脑室内注射干扰素-γ的大鼠脑内免疫反应的分布和时间调节
DOI：
10.1006/exnr.1998.6943
复制
发表时间：
1998
影响因子：
5.3
通讯作者：
M. Bentivoglio
中科院分区：
文献类型：
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作者：
Ze;K. Kristensson;M. Bentivoglio
本文观察了成年Wistar大鼠脑室内注射干扰素-γ的反应：对注射生理盐水1周后的大鼠进行了主要组织相容性复合体(MHC)I、II类抗原、CD8和CD4抗原以及巨噬细胞/小胶质细胞抗原OX42的分析。双标记法鉴定表达MHC抗原的神经胶质细胞类型。因此，经番茄凝集素组织化学鉴定，干扰素-γ可诱导小胶质细胞表达MHC-I和MHC-II，而对GFAP免疫染色的星形胶质细胞则无此作用。MHC抗原表达的小胶质细胞见于脑室周围实质、大脑皮层、小脑、主要纤维束和脑干浅层实质的多个区域。在这些区域，MHC免疫阳性成分的密度和染色强度不同，其中MHC I类抗原持续1周，而MHC II类诱导下降。定量分析表明，脑室周围和基底脑区有OX42免疫反应阳性细胞的增殖。注射干扰素-γ后3d，脑室周围、基底前脑和纤维束内可见CD8+T细胞，7d后CD8+T细胞密度明显增加。CD_4~+T细胞明显少于CD_8~+T细胞。但在脑室周围和软膜下可见大量的小胶质细胞，尤以注射干扰素-γ后1周更为明显。我们的数据表明，这种促炎细胞因子在体内介导了小胶质细胞的激活和脑内T细胞的渗透，参与这一免疫反应的细胞表现出区域选择性和不同的抗原表达时间调节。
The response to intracerebroventricular administration of interferon (IFN)-gamma was examined in the adult Wistar rat brain: major histocompatibility complex (MHC) antigens class I and II, CD8 and CD4 antigens, and the macrophage/microglia antigen OX42 were analyzed in respect to saline-injected cases over 1 week. The glial cell type expressing MHC antigens was characterized with double labeling. IFN-gamma was thus found to induce MHC class I and II expression in microglia, identified by tomato lectin histochemistry, and not in GFAP-immunostained astrocytes. MHC antigen-expressing microglia was detected in the periventricular parenchyma, several fields of the cerebral cortex, cerebellum, major fiber tracts, and brainstem superficial parenchyma. Different gradients of density and staining intensity of the MHC-immunopositive elements were observed in these regions, in which MHC class I antigens persisted up to 1 week, when MHC class II induction had declined. Quantitative analysis pointed out the proliferation of OX42-immunoreactive cells in periventricular and basal brain regions. CD8+ T cells were observed in periventricular regions, basal forebrain, and fiber tracts 3 days after IFN-gamma injection and their density markedly increased by 7 days. CD4+ T cells were also seen and they were fewer than CD8+ ones. However, numerous CD4+ microglial cells were observed in periventricular and subpial regions, especially 1 week after IFN-gamma injection. Our data indicate that this proinflammatory cytokine mediates in vivo microglia activation and T cell infiltration in the brain and that the cells involved in this immune response display a regional selectivity and a different temporal regulation of antigen expression.
自身免疫性疾病和神经系统。
DOI：
--
发表时间：
1992
期刊：
The Western journal of medicine
影响因子：
--
作者：
Merrill,JE;Graves,MC;Mulder,DG
通讯作者：
Mulder,DG
对人脑活检中 MHC 表达的单克隆抗体分析：从“组织学正常”到显示不同程度的神经胶质瘤参与的组织。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lampson,LA;Hickey,WF
通讯作者：
Hickey,WF