l-Glutamine and l-arginine protect against enterotoxigenic Escherichia coli infection via intestinal innate immunity in mice
l-Glutamine and l-arginine protect against enterotoxigenic Escherichia coli infection via intestinal innate immunity in mice
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L-谷氨酰胺和L-精氨酸通过肠道先天免疫保护小鼠免受产肠毒素大肠杆菌感染
DOI：
10.1007/s00726-017-2410-9
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发表时间：
2017-12-01
期刊：
影响因子：
3.5
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Liu, Gang;Ren, Wenkai;Yin, Yulong
饮食中补充谷氨酰胺（Gln）或精氨酸（Arg）有益于肠道健康;然而，Gln或Arg是否可以保护肠道免受产肠毒素大肠杆菌（ETEC）感染尚不清楚。为了解决这个问题，我们使用ETEC感染的小鼠模型来研究Gln和Arg的保护作用。在实验中，我们在口服ETEC感染前用设计的Gln或Arg补充饲料预处理小鼠，然后评估小鼠死亡率和肠道细菌负荷。我们还确定了治疗小鼠肠道先天免疫的标志物，包括分泌型伊加反应（SIgA），杯状细胞的粘蛋白，以及潘氏细胞的抗菌肽。ETEC在小鼠小肠（包括十二指肠、空肠和回肠）中定殖，并抑制肠道免疫因子（如多聚免疫球蛋白受体（pIgR）、cryptdin相关序列1C（CRS 1C）和Reg 3 γ）的mRNA表达。我们发现饮食中补充Gln或Arg减少了细菌定植并促进了先天免疫的激活（例如，ETEC感染小鼠肠道中pIgR、CRS 1C和Reg 3 γ）的mRNA表达。我们的研究结果表明，饮食中补充精氨酸或谷氨酰胺可能通过肠道先天免疫抑制肠道ETEC感染。
Dietary glutamine (Gln) or arginine (Arg) supplementation is beneficial for intestinal health; however, whether Gln or Arg may confer protection against Enterotoxigenic Escherichia coli (ETEC) infection is not known. To address this, we used an ETEC-infected murine model to investigate the protective effects of Gln and Arg. Experimentally, we pre-treated mice with designed diet of Gln or Arg supplementation prior to the oral ETEC infection and then assessed mouse mortality and intestinal bacterial burden. We also determined the markers of intestinal innate immunity in treated mice, including secretory IgA response (SIgA), mucins from goblet cells, as well as antimicrobial peptides from Paneth cells. ETEC colonized in mouse small intestine, including duodenum, jejunum, and ileum, and inhibited the mRNA expression of intestinal immune factors, such as polymeric immunoglobulin receptor (pIgR), cryptdin-related sequence 1C (CRS1C), and Reg3γ. We found that dietary Gln or Arg supplementation decreased bacterial colonization and promoted the activation of innate immunity (e.g., the mRNA expression of pIgR, CRS1C, and Reg3γ) in the intestine of ETEC-infected mice. Our results suggest that dietary arginine or glutamine supplementation may inhibit intestinal ETEC infection through intestinal innate immunity.