Identification of cytochrome P-450, and its distribution in the membrana granulosa of the preovulatory follicle, using quantitative cytochemistry.
Identification of cytochrome P-450, and its distribution in the membrana granulosa of the preovulatory follicle, using quantitative cytochemistry.
复制标题
使用定量细胞化学鉴定细胞色素 P-450 及其在排卵前卵泡颗粒膜中的分布。
作者：
L. C. Zoller;J. Weisz
用定量细胞化学方法测定了动情前期大鼠排卵前卵泡冰冻切片中细胞色素P-450（C-P450）的分布。在加入或不加入连二亚硫酸钠的情况下，使这些部分与用一氧化碳饱和的介质反应。在400至500 nm处测量吸收光谱，并通过从存在连二亚硫酸钠的情况下获得的消光值中减去不含连二亚硫酸钠的孵育物获得的消光值来计算差异光谱。在颗粒膜（MG）的外周部分的细胞中记录到在450 nm处的一个明显的峰，但在卵丘的细胞中没有记录到，这为排卵前卵泡MG中C-P450的存在和差异分布提供了证据。
The distribution of cytochrome P-450 (C-P450), an essential component of the oxidative enzyme system involved in the hydroxylation of steroids, was measured by quantitative cytochemistry in cryostat sections of preovulatory follicles obtained from rats in proestrous. The sections were reacted with medium saturated with carbon monoxide with or without the addition of sodium dithionite. The absorbance spectrum was measured from 400 to 500 nm and a difference spectrum calculated by subtracting the extinction obtained from incubations without sodium dithionite from that obtained in the presence of sodium dithionite. A distinct peak at 450nm was recorded in cells of the peripheral portion of the membrana granulosa (MG) but not in those of the cumulus, providing evidence for the presence and differential distribution of C-P450 in the MG of the preovulatory follicle.