Identification of dengue type 2 virus-specific high molecular weight proteins in virus-infected BHK cells.
Identification of dengue type 2 virus-specific high molecular weight proteins in virus-infected BHK cells.
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DOI：
10.1099/0022-1317-66-12-2767
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发表时间：
1985-12
期刊：
影响因子：
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通讯作者：
G. R. Cleaves
中科院分区：
文献类型：
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作者：
G. R. Cleaves
有证据表明，登革热 2 型病毒 (DEN-2) 感染的 BHK 细胞中会产生分子量高达 250 000 的大型病毒特异性蛋白质。这些蛋白质在用 [35S] 蛋氨酸标记 7 至 15 分钟的细胞裂解物中最为突出。在脉冲追踪实验中，这些高分子。重量。蛋白质似乎被转化为更小、更稳定的蛋白质。重量。 10 000 至 98 000 之间。最后，蛋白水解的抑制阻止了对高分子量标记的追逐。重量。蛋白质转化为通常在 DEN-2 感染细胞中积累的较小病毒蛋白质。这些发现与大多蛋白前体的加工在黄病毒蛋白的生产中发挥重要作用的观点是一致的。
Evidence is presented for the production in dengue type 2 virus (DEN-2)-infected BHK cells of large virus-specific proteins with molecular weights up to 250 000. These proteins were most prominent in lysates of cells which had been labelled with [35S]methionine for 7 to 15 min. During pulse-chase experiments, these high mol. wt. proteins appeared to be converted into smaller, more stable, proteins with mol. wt. between 10 000 and 98 000. Finally, inhibition of proteolysis prevented the chasing of label from the high mol. wt. proteins to the smaller viral proteins which normally accumulate in DEN-2-infected cells. These findings are consistent with the idea that processing of large polyprotein precursors plays an important role in the production of flavivirus proteins.