A retrospective metagenomics approach to studying Blastocystis
A retrospective metagenomics approach to studying Blastocystis
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DOI：
10.1093/femsec/fiv072
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发表时间：
2015-07-01
影响因子：
4.2
通讯作者：
Stensvold, Christen Rune
中科院分区：
文献类型：
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作者：
Andersen, Lee O'Brien;Bonde, Ida;Stensvold, Christen Rune
芽囊原虫是一种常见的单细胞肠道寄生虫属，由几个亚型组成。在这里，我们筛选了通过对芽囊原虫粪便DNA进行元基因组分析所获得的数据，方法是在共丰度基因组中搜索亚型特有的基因，这些基因组在选择的316个人类粪便样本中存在共同差异，因此代表来自单一亚型的基因。316份粪便标本来自236例健康人、13例克罗恩病(CD)和67例溃疡性结肠炎(UC)患者。健康人的芽囊原虫患病率为20.3%，UC患者的患病率为14.9%。同时，CD患者中未见芽囊原虫。肠道微生物群以类杆菌为主的个体比瘤胃球菌和普氏杆菌驱动的肠型个体更不容易出现芽囊原虫阳性粪便(马太相关系数=-0.25P<；0.0001)。这是第一次使用元基因组学方法研究芽囊原虫和肠道细菌群落之间的关系。这项研究是一个例子，说明如何使用针对肠道微生物组的细菌成分以及这些微生物群落之间的相互作用的元基因组数据集，回顾调查肠道中的微生物真核生物群落。
Blastocystis is a common single-celled intestinal parasitic genus, comprising several subtypes. Here, we screened data obtained by metagenomic analysis of faecal DNA for Blastocystis by searching for subtype-specific genes in coabundance gene groups, which are groups of genes that covary across a selection of 316 human faecal samples, hence representing genes originating from a single subtype. The 316 faecal samples were from 236 healthy individuals, 13 patients with Crohn's disease (CD) and 67 patients with ulcerative colitis (UC). The prevalence of Blastocystis was 20.3% in the healthy individuals and 14.9% in patients with UC. Meanwhile, Blastocystis was absent in patients with CD. Individuals with intestinal microbiota dominated by Bacteroides were much less prone to having Blastocystis-positive stool (Matthew's correlation coefficient = -0.25, P < 0.0001) than individuals with Ruminococcus-and Prevotella-driven enterotypes. This is the first study to investigate the relationship between Blastocystis and communities of gut bacteria using a metagenomics approach. The study serves as an example of how it is possible to retrospectively investigate microbial eukaryotic communities in the gut using metagenomic datasets targeting the bacterial component of the intestinal microbiome and the interplay between these microbial communities.