Structural and functional conservation of the Caenorhabditis elegans timing gene clk-1
Structural and functional conservation of the Caenorhabditis elegans timing gene clk-1
复制标题
DOI：
10.1126/science.275.5302.980
复制
发表时间：
1997-02-14
期刊：
影响因子：
56.9
通讯作者：
Hekimi, S
中科院分区：
文献类型：
--
作者：
Ewbank, JJ;Barnes, TM;Hekimi, S
秀丽隐杆线虫基因clk-1的突变会影响生物计时，延长寿命。克隆的CLK-1基因与CLK-1的表型互补，恢复了正常寿命。CLK-1蛋白在包括人类在内的真核生物中被发现是保守的，并且在结构上与酵母代谢调节因子Cat5p(也称为Coq7p)相似。这些蛋白质包含一个核心82个残基结构域的串联复制。CLK-1与CAT5/COQ7缺失突变体的表型互补，说明CLK-1与CAT5/COQ7具有共同的生化功能，CLK-1在细胞生理水平发挥作用。
Mutations in the Caenorhabditis elegans gene clk-1 affect biological timing and extend longevity. The gene clk-1 was identified, and the cloned gene complemented the clk-1 phenotypes and restored normal longevity. The CLK-1 protein was found to be conserved among eukaryotes, including humans, and structurally similar to the yeast metabolic regulator Cat5p (also called Coq7p). These proteins contain a tandem duplication of a core 82-residue domain. clk-1 complemented the phenotype of cat5/coq7 null mutants, demonstrating that clk-1 and CAT5/COQ7 share biochemical function and that clk-1 acts at the level of cellular physiology.