Lipolysis and cAMP accumulation in adipocytes in response to physical training.
Lipolysis and cAMP accumulation in adipocytes in response to physical training.
复制标题
身体训练引起的脂肪细胞中的脂肪分解和 cAMP 积累。
DOI：
10.1152/jappl.1981.50.1.143
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Gollnick,PD
中科院分区：
文献类型：
--
作者：
Shepherd,RE;Noble,EG;Klug,GA;Gollnick,PD
与久坐不动的大鼠相比，运动训练的脂肪细胞匀浆的膜制剂中的腺苷环化酶活性较低(J.Appl。呼吸。艾维隆，锻炼身体。1977年，42：884-888)。在本研究中，我们测定了来自安静和训练的大鼠子宫旁脂肪细胞的脂解作用和环腺苷3‘：5’-单磷酸(CAMP)的积聚。这些研究的目的是确定耐力训练大鼠分离的脂肪细胞中，正常儿茶酚胺诱导的cAMP积累增加是否受到影响。在训练大鼠分离的脂肪细胞中，异丙肾上腺素(0.01-10微米)引起的cAMP积聚增加减少。然而，在这些脂肪细胞中，对相同荷尔蒙挑战的甘油释放更多。在0.125和1.025微米处测得的cAMP磷酸二酯酶活性，训练组大鼠脂肪细胞匀浆颗粒部分中cAMP磷酸二酯酶活性高于安静组。如果动物在休息状态下被杀死，那么来自训练大鼠的粗脂肪垫匀浆制剂中激素敏感的脂肪酶活性会降低。然而，如果动物在被杀前立即跑到筋疲力尽，那么训练过的和未训练过的大鼠的制剂中激素敏感脂肪酶的活性没有差异。这些数据表明，尽管在训练的大鼠中，脂肪细胞对异丙肾上腺素的反应中cAMP的积累明显减少，但脂肪分解和激素敏感脂肪酶的激活并没有减少。
Adenylate cyclase activity is lower in membrane preparations of fat cell homogenates from exercise-trained compared with sedentary rats (J. Appl. Physiol.: Respirat. Environ, Exercise Physiol. 42: 884-888, 1977). In the present investigations lipolysis and cyclic adenosine 3':5'-monophosphate (cAMP) accumulation were measured in isolated parametrial fat cells prepared from sedentary and trained rats. The purpose of these investigations was to determine whether the normal catecholamine-induced increases in cAMP accumulation is affected in isolated adipocytes from endurance-trained rats. The increases in cAMP accumulation in response to isoproterenol (0.01-10 microM) was reduced in fat cells isolated from trained rats. However, glycerol release in response to the same hormonal challenge was greater in these adipocytes. cAMP phosphodiesterase activity measured at 0.125 and 1.025 microM cAMP was greater in the particulate fraction of fat cell homogenates obtained from trained rats as compared with their sedentary counterparts. Hormone-sensitive lipase activity was reduced in crude fat pad homogenate preparations from trained rats if the animals were killed at rest. However, if the animals were run to exhaustion immediately prior to being killed, there were no differences in the hormone-sensitive lipase activity between preparations from trained and nontrained rats. These data indicate that, although cAMP accumulation by isolated fat cells in response to isoproterenol is markedly lower in trained rats, lipolysis and hormone-sensitive lipase activation is not reduced.