A submillimeter resolution fluorescence molecular imaging system for small animal imaging
A submillimeter resolution fluorescence molecular imaging system for small animal imaging
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DOI：
10.1118/1.1568977
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发表时间：
2003-05-01
期刊：
影响因子：
3.8
通讯作者：
Ntziachristos, V
中科院分区：
文献类型：
--
作者：
Graves, EE;Ripoll, J;Ntziachristos, V
目前为利用漫反射光子对完整组织进行层析成像研究而开发的大多数成像系统都受到光源和探测器数量的限制。本文描述了一种用于小动物荧光成像的大数据集、低噪声层析成像系统的构建和评价。该系统由平行板-成像室和透镜耦合的CCD摄像机组成，可以进行常规的平面成像和荧光层析成像。平面成像数据用于指导获取包含超过106个测量值的荧光分子断层扫描(FMT)数据集，并将解剖特征与断层扫描结果叠加以改善视觉表示。实验测量结果与用于预测光在腔内传播的扩散理论模型很好地吻合。对该仪器定量重建三维荧光分布的测试表明，实际荧光浓度与FMT结果之间的误差不到5%，并建议对小型局部对象的检测阈值约为100 femptomoles。评估该仪器空间分辨率的实验证明，该系统能够分辨放置在净距离小于1毫米的物体。这比以前开发的动物成像系统的分辨率有了显著的提高，这主要是因为使用了大量的数据集和使用了反演方法。最后，展示了在体成像的能力。预计收集的大数据集可以使分子探针在体内获得更好的成像，并提高荧光信号的量化。(C)2003年美国医学物理学家协会。
Most current imaging systems developed for tomographic investigations of intact tissues using diffuse photons suffer from a limited number of sources and detectors. In this paper we describe the construction and evaluation of a large dataset, low noise tomographic system for fluorescence imaging in small animals. The system consists of a parallel plate-imaging chamber and a lens coupled CCD camera, which enables conventional planar imaging as well as fluorescence tomography. The planar imaging data are used to guide the acquisition of a Fluorescence Molecular Tomography (FMT) dataset containing more than 106 measurements, and to superimpose anatomical features with tomographic results for improved visual representation. Experimental measurements exhibited good agreement with the diffusion theory models used to predict light propagation within the chamber. Tests of the instrument's capacity to quantitatively reconstruct fluorochrome distributions in three dimensions showed less than 5% errors between actual fluorochrome concentrations and FMT findings, and suggested a detection threshold of approximately 100 femptomoles for small localized objects. Experiments to assess the instrument's spatial resolution demonstrated the ability of the system to resolve objects placed at clear distances of less than 1 mm. This is a significant resolution increase over previously developed systems for animal imaging, and is primarily due to the large dataset employed and the use of inversion methods. Finally, the in vivo imaging capacity is showcased. It is expected that the large dataset collected can enable superior imaging of molecular probes in vivo and improve quantification of fluorescence signatures. (C) 2003 American Association of Physicists in Medicine.