In-situ Visualization and SERS monitoring of the interaction between tumor and endothelial cells using 3D microfluidic networks.
In-situ Visualization and SERS monitoring of the interaction between tumor and endothelial cells using 3D microfluidic networks.
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DOI：
10.1021/acssensors.9b02085
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发表时间：
2019-12
期刊：
影响因子：
8.9
通讯作者：
Ziting Qian;Jiayuan Fei;S. Zong;Kuo Yang;Lang Li;Ruiyang Liu;Zhuyuan Wang;Yiping Cui
中科院分区：
文献类型：
--
作者：
Ziting Qian;Jiayuan Fei;S. Zong;Kuo Yang;Lang Li;Ruiyang Liu;Zhuyuan Wang;Yiping Cui
一种多功能微流控平台通过集成三维（3D）细胞培养单元和蛋白质检测单元来监测肿瘤细胞和内皮细胞之间的相互作用。在该芯片中，将乳腺癌MCF7细胞植入胶原中形成3D肿瘤环境，将人脐静脉内皮细胞（HUVEC）植入胶原基质旁边的通道中。因此，MCF7与HUVEC共培养4天后，在三维胶原基质中可以通过荧光观察到血管新生芽的原位生长，而在二维培养环境中则无法观察到。另一方面，利用gold@silver核壳纳米棒作为SERS免疫探针，检测细胞因子（血管内皮生长因子，VEGF）的分泌。VEGF的检出限为100 pg/mL。此外，由于LiCl和贝伐单抗可以作为VEGF的启动子和抑制剂，因此可以通过SERS信号监测它们刺激下VEGF浓度的动态变化。因此，这种集成的SERS微流控平台为肿瘤与内皮细胞相互作用的基础研究创造了机会。
A multifunctional microfluidic platform was demonstrated to monitor the interaction between tumor cells and endothelial cells by integrating a three-dimension (3D) cell culture unit with a protein detection unit. In such a chip, breast cancer cells of MCF7 were seeded into the collagen to form a 3D tumor environment while human umbilical vein endothelial cells (HUVEC) are seeded in the channel next to the collagen matrix. Thus, an in-situ growth of angiogenic sprouting can be visualized through fluorescence in the 3D collagen matrix after a co-culture of MCF7 and HUVEC after four days, which cannot be observed in the 2D culture environment. On the other hand, gold@silver core-shell nanorods were used as SERS immunoprobes for the detection of the secretion of cytokine (vascular endothelial growth factor, VEGF). The limit of detection of the VEGF is 100 pg/mL. Further, as LiCl and bevacizumab can act as a promoter and an inhibitor of VEGF, the dynamic change of the concentration of VEGF under the stimulation of them was monitored by SERS signals. Thus, this integrated SERS microfluidic platform creates opportunity for the fundamental research of interaction between tumors and endothelial cells.