Comprehensive analysis of the percentage of surface receptors and cytotoxic granules positive natural killer cells in patients with pancreatic cancer, gastric cancer, and colorectal cancer.
Comprehensive analysis of the percentage of surface receptors and cytotoxic granules positive natural killer cells in patients with pancreatic cancer, gastric cancer, and colorectal cancer.
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胰腺癌、胃癌、结直肠癌患者表面受体及细胞毒颗粒阳性自然杀伤细胞百分比综合分析
DOI：
10.1186/1479-5876-11-262
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发表时间：
2013-10-20
影响因子：
7.4
通讯作者：
Miao Y
中科院分区：
文献类型：
--
作者：
Peng YP;Zhu Y;Zhang JJ;Xu ZK;Qian ZY;Dai CC;Jiang KR;Wu JL;Gao WT;Li Q;Du Q;Miao Y
背景消化道恶性肿瘤，尤其是胰腺癌（PC）、胃癌（GC）和结直肠癌（CRC）的发生率仍然持续很高，并且这些癌症的疾病进展与肿瘤免疫监视逃逸有关。自然杀伤（NK）细胞功能障碍可能是造成这种现象的原因，然而，消化道恶性肿瘤中肿瘤免疫监视逃逸与NK细胞功能障碍之间的确切关系尚不清楚。并对胰腺癌（n = 31）、胃癌（n = 31）、和CRC（n = 32）和健康对照（n = 31）。采用独立检验或Mann-Whitney U检验比较患者与健康对照组之间的差异，以及不同病理特征的癌症患者之间的差异。结果与健康对照组相比，PC患者以及GC和CRC患者的NKG 2D、NKp 30、NKp 46和穿孔素阳性NK细胞百分比显著下调;这些分子水平的降低与每种恶性肿瘤中疾病进展的指标（如组织学分级、浸润深度、淋巴结转移）相关。相反，KIR 3DL 1阳性NK细胞的百分比显着增加，在PC患者，以及GC和CRC，但不与任何指标的疾病progression.ConclusionsAltered百分比的表面受体和细胞毒性颗粒阳性NK细胞可能发挥重要作用，在肿瘤免疫监视逃避诱导NK细胞功能障碍的PC，GC和CRC患者。
BackgroundDigestive malignancies, especially pancreatic cancer (PC), gastric cancer (GC), and colorectal cancer (CRC), still occur at persistently high rates, and disease progression in these cancers has been associated with tumor immunosurveillance escape. Natural killer (NK) cell dysfunction may be responsible for this phenomenon, however, the exact relationship between tumor immunosurveillance escape in digestive malignancies and NK cell dysfunction remains unclear.MethodsPercentage of the surface receptors NKG2A, KIR3DL1, NKG2D, NKp30, NKp44, NKp46, and DNAM-1, as well as the cytotoxic granules perforin and granzyme B positive NK cells were determined in patients with pancreatic cancer (n = 31), gastric cancer (n = 31), and CRC (n = 32) prior to surgery and healthy controls (n = 31) by multicolor flow cytometry. Independentt-tests or Mann-Whitney U-tests were used to compare the differences between the patient and healthy control groups, as well as the differences between patients with different pathologic features of cancer.ResultsPercentage of NKG2D, NKp30, NKp46, and perforin positive NK cells was significantly down-regulated in patients with PC compared to healthy controls, as well as GC and CRC; reduced levels of these molecules was associated with indicators of disease progression in each malignancy (such as histological grade, depth of invasion, lymph node metastasis). On the contrary, percentage of KIR3DL1 positive NK cells was significantly increased in patients with PC, as well as GC and CRC, but was not associated with any indicators of disease progression.ConclusionsAltered percentage of surface receptors and cytotoxic granules positive NK cells may play a vital role in tumor immunosurveillance escape by inducing NK cell dysfunction in patients with PC, GC, and CRC.
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DOI：
10.1158/1078-0432.ccr-09-0991
发表时间：
2009-11-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
McGilvray RW;Eagle RA;Watson NF;Al-Attar A;Ball G;Jafferji I;Trowsdale J;Durrant LG
通讯作者：
Durrant LG
影响因子：
15.9
作者：
Mamessier, Emilie;Sylvain, Aude;Olive, Daniel
通讯作者：
Olive, Daniel
影响因子：
20.3
作者：
Correia, Daniel V.;Fogli, Manuela;Silva-Santos, Bruno
通讯作者：
Silva-Santos, Bruno
影响因子：
4.4
作者：
Konjevic, Gordana;Jurisic, Vladimir;Spuzic, Ivan
通讯作者：
Spuzic, Ivan
影响因子：
3.4
作者：
Duan, Xiaohui;Deng, Langmei;Sun, Weijia
通讯作者：
Sun, Weijia