Polydopamine-Assisted Anchor of Chitosan onto Porous Composite Scaffolds for Accelerating Bone Regeneration
Polydopamine-Assisted Anchor of Chitosan onto Porous Composite Scaffolds for Accelerating Bone Regeneration
复制标题
聚多巴胺辅助壳聚糖锚定在多孔复合支架上以加速骨再生
DOI：
10.1021/acsbiomaterials.9b00209
复制
发表时间：
2019-06-01
影响因子：
5.8
通讯作者：
Liao, Gui-Qing
中科院分区：
文献类型：
--
作者：
Li, Xiang;Yin, Hua-Mo;Liao, Gui-Qing
表面功能对多孔聚合物支架的生物活性具有重要意义。本研究的目的是将具有高生物活性的壳聚糖（CS）固定在多孔复合支架表面以加速骨再生。通过受贻贝启发的聚多巴胺（PDA）涂层作为桥接层，制备了具有牢固壳聚糖锚定的多孔聚（ε - 己内酯）（PCL）/生物活性玻璃（BG）复合支架。体外细胞培养表明，与通过物理吸附修饰壳聚糖的支架相比，将壳聚糖牢固地固定在复合支架上显著增强了蛋白质吸附、细胞黏附和成骨分化。体内评估表明，与壳聚糖物理吸附的对应物相比，将壳聚糖共价固定在支架表面明显促进了颅骨再生。所提出的方法为制造用于骨组织工程应用的人工生物活性支架提供了一种可行且有效的手段。
Surface function has an importance for the bioactivity of porous polymeric scaffolds. The goal of the present study is to immobilize highly bioactive chitosan (CS) onto the surface of porous composite scaffolds to accelerate bone regeneration. Porous poly(epsilon-caprolactone) (PCL)/bioactive glass (BG) composite scaffolds with strong anchor of CS were fabricated via mussel-inspired polydopamine (PDA) coating as a bridging layer. In vitro cell culture showed that firm immobilization of CS onto the composite scaffolds significantly enhanced protein adsorption, cell adhesion, and osteogenic differentiation compared to CS-decorated scaffolds via physical adsorption. In vivo assessments demonstrated that covalent immobilization of CS onto the surface of scaffolds obviously promoted cranial bone regeneration in comparison with the counterparts with physical adsorption of CS. The proposed method offers a feasible and effective means to fabricate artificial bioactive scaffolds for bone tissue engineering application.