Identification and localization of soluble sulfotransferases in the human gastrointestinal tract
Identification and localization of soluble sulfotransferases in the human gastrointestinal tract
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DOI：
10.1042/bj20061431
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发表时间：
2007-06-01
影响因子：
4.1
通讯作者：
Glatt, Hansruedi
中科院分区：
文献类型：
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作者：
Teubner, Wera;Meinl, Walter;Glatt, Hansruedi
可溶性SULT（磺基转移酶）在信使分子的调节和异生物质的消除中是重要的。然而，各种底物的磺基缀合也可导致反应性代谢物的形成，所述反应性代谢物可诱发癌症并引起其他损害。本研究的目的是鉴定在人类胃肠道，特别是结肠和直肠（常见的癌症部位）中表达的SULT形式，并确定其细胞定位。正常的结肠或直肠组织，切除肿瘤，从39名受试者。为了比较，我们另外研究了来自胃、空肠、回肠、盲肠和肝脏的一到四个样品。通过免疫印迹、免疫组织化学和酶活性测定检测SULT。SULT 1A1、1A3和1B1存在于胃肠道的所有部分，通常超过肝脏中的水平（这些形式分别以高、不可检测和低水平存在）。它们主要位于分化的肠上皮细胞中。SULT1E1和2A1仅在肝脏、空肠、回肠和盲肠中检测到。SULT1C1很容易在胃中发现，但在其他地方可以忽略不计。SULT1A2在个体样品中以低水平存在。未检测到其余形式，限制是使用抗血清只能识别高水平。总之，SULT在人类胃肠道中含量丰富，我们推测它们参与了消化道微生物释放的生物活性食源性成分（包括糖苷配基）的系统前消除，以及某些前致癌物的生物活化。
Soluble SULTs (sulfotransferases) are important in the regulation of messenger molecules and the elimination of xenobiotics. However, sulfo-conjugation of various substrates can also lead to the formation of reactive metabolites that may induce cancer and cause other damage. The aim of the present study was to identify the SULT forms expressed in the human gastrointestinal tract, especially the colon and rectum (common sites for cancer), and to determine their cellular localization. Normal colonic or rectal tissue, resected with tumours, was obtained from 39 subjects. For comparison, we additionally studied one to four samples from stomach, jejunum, ileum, cecum and liver. SULTs were detected by immunoblotting, immunohistochemistry and measurement of enzyme activities. SULT1A1, 1A3 and 1B1 were found in all parts of the gastrointestinal tract, often exceeding levels in liver (where these forms were present at high, undetectable and low levels respectively). They were predominantly localized in differentiated enterocytes. SULT1E1 and 2A1 were only detected in liver, jejunum, ileum and cecum. SULT1C1 was readily found in stomach, but was negligible elsewhere. SULT1A2 was present at low levels in individual samples. The remaining forms were not detected with the limitation that only high levels could be recognized with the antisera used. In conclusion, SULTs are abundant in the gastrointestinal tract of man. We suspect that they are involved in the presystemic elimination of bioactive food-borne components, including aglycones released by gut microbiota, as well as the bioactivation of some procarcinogens.