Determination of quercetin, plumbagin and total flavonoids in Drosera peltata Smith var. glabrata Y.Z.Ruan.
Determination of quercetin, plumbagin and total flavonoids in Drosera peltata Smith var. glabrata Y.Z.Ruan.
复制标题
茅膏菜中槲皮素、白花丹素和总黄酮的测定
DOI：
10.4103/0973-1296.103649
复制
发表时间：
2012-10
影响因子：
0.7
通讯作者：
Wan H
中科院分区：
文献类型：
--
作者：
He Y;He Z;He F;Wan H
牡丹是牡丹科野生肉食性植物，民间常用于治疗风湿病和瘀伤。在以前的研究中，这种草药中的化合物都没有被量化。建立高效液相色谱法同时测定槲皮素和白丹素两种生物活性成分的方法，并建立简便的紫外分光光度法测定白丹总黄酮含量的方法。色谱分离采用Agilent Eclipse XDB C18色谱柱和乙腈-水（含0.1%磷酸，V/V）梯度洗脱体系组成的高效液相色谱系统，分离时间为20分钟。将槲皮素配合物与Al(NO3)3进行比较，采用269 nm紫外分光光度法测定其总黄酮含量。两种方法均具有良好的线性度（高效液相色谱法槲皮素和白丹素的线性度r2≥0.9994，紫外分光光度法槲皮素和白丹素的线性度r2 = 0.9994）、精密度（高效液相色谱法槲皮素和白丹素的日内变异性分别小于0.738%和1.64%，紫外分光光度法槲皮素和白丹素的日变异性分别小于1.67%）和加样回收率（槲皮素和白丹素的HPLC法加样回收率分别为96.7-100.4%和99.4 -100.4%）。紫外分光光度法测定槲皮素的回收率为96.7 ~ 99.6%。)该方法简便、准确，可用于中药材中槲皮素、白丹素和总黄酮的快速测定，为中药材的质量控制提供了有效的方法。
Drosera peltata Smith var. glabrata Y.Z.Ruan, a kind of wild carnivorous plants in the family Droseraceae, has been used for the treatment of rheumatism and bruises in Chinese folk. None of compounds in this herb has been quantified in the previous studies. To develop a validated and reliable HPLC method for the simultaneous determination of two bioactive constituents – quercetin and plumbagin, and establish a simple UV spectrophotometry method for the analysis of total flavonoids content. Chromatographic separation was performed by using a HPLC system consisting of an Agilent Eclipse XDB C18 column and a gradient elution system of acetonitrile and water (containing 0.1% phosphoric acid, V/V) within 20 minutes. Comparing with quercetin complex with Al(NO3)3, the total flavonoids were determined by UV spectrophotometry at 269 nm. Both methods were validated for linearity (r2≥0.9994 for quercetin and plumbagin in the HPLC method, r2 = 0.9994 for quercetin in the UV spectrophotometry method), precision (The within-day and between-day variability was less than 0.738% and 1.64% for quercetin and plumbagin in the HPLC method, and was less than 1.67% for quercetin in the UV spectrophotometry method.) and recovery (The recoveries of the HPLC method were 96.7-100.4% and 97.4-100.4% for quercetin and plumbagin, respectively, and the recovery of the UV spectrophotometry method was 96.7-99.6% for quercetin.) The proposed methods are simple and accurate, and could be practiced to rapidly determine quercetin, plumbagin and total flavonoids in the herbal drug, which provide effective approaches for quality control.
影响因子：
0.7
作者：
Chen Y;Wang J;Wan D
通讯作者：
Wan D
DOI：
10.1124/jpet.105.098863
发表时间：
2006-08-01
影响因子：
3.5
作者：
Hsu, Ya-Ling;Cho, Chien-Yu;Lin, Chun-Ching
通讯作者：
Lin, Chun-Ching