Genetic characterization of Trypanosoma brucei gambiense and clinical evolution of human African trypanosomiasis in Cote d'Ivoire
Genetic characterization of Trypanosoma brucei gambiense and clinical evolution of human African trypanosomiasis in Cote d'Ivoire
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DOI：
10.1046/j.1365-3156.2002.00905.x
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发表时间：
2002-07-01
影响因子：
3.3
通讯作者：
Truc, P
中科院分区：
文献类型：
--
作者：
Jamonneau, V;Garcia, A;Truc, P
人类非洲锥虫病是一种由属于布氏锥虫亚种的原生动物引起的寄生虫感染。这种疾病的临床演变是复杂的，可能是因为寄生虫本身，因为在T。布氏亚种我们调查了锥虫的遗传多样性和临床模式在科特迪瓦的多样性之间的关系。我们研究了临床昏睡病病例，并对从这些患者中分离的锥虫进行了遗传分析。通过同工酶电泳、随机扩增多态性DNA和微卫星序列PCR等多种标记，观察到科特迪瓦分离的种群之间存在重要的遗传单态性。同时，临床分析证实了临床模式和演变的多样性。个体对疾病的易感性（人类锥虫耐受性）的存在应该被考虑在内，即使我们的遗传学结论可能会被扭曲，因为分离成功率特别低。事实上，我们观察到，隔离成功率的差异显着取决于原产地（P = 0.0002）和种族群体（P = 0.0317）的病人。需要进一步研究，以研究使用锥虫体外分离试剂盒作为分离技术可能产生的选择性影响。
Human African trypanosomiasis is a parasitic infection caused by protozoa belonging to Trypanosoma brucei subspecies. The clinical evolution of this disease is complex and might be because of the parasite itself, as genetic diversity has been observed in T. brucei ssp. We investigated the relationship between the genetic diversity of trypanosomes and the diversity of clinical patterns in Cote d'Ivoire. We studied clinical sleeping sickness cases, and genetically analysed the trypanosomes isolated from these patients. An important genetic monomorphism among stocks isolated in Cote d'Ivoire was observed by using various markers: isoenzymes electrophoresis, random amplified polymorphism DNA and PCR of microsatellite sequences. At the same time, the diversity of clinical patterns and evolutions was confirmed by clinical analysis. The existence of an individual susceptibility to disease (human trypanotolerance) should be taken into account even if our genetic conclusions might be distorted because the isolation success rates were particularly poor. In fact, we observed that the isolation success rate varied significantly depending both on the focus of origin (P = 0.0002) and on the ethnic group (P = 0.0317) of the patient. Further investigations are required in order to study a possible selective impact of the use of the kit for in vitro isolation of trypanosomes as an isolation technique.