A guanine nucleotide-dependent regulatory protein couples substance P receptors to phospholipase C in rat parotid gland.
A guanine nucleotide-dependent regulatory protein couples substance P receptors to phospholipase C in rat parotid gland.
复制标题
鸟嘌呤核苷酸依赖性调节蛋白将大鼠腮腺中的 P 物质受体与磷脂酶 C 偶联。
DOI：
10.1016/0006-291x(86)90919-8
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Rubin,RP
中科院分区：
文献类型：
--
作者：
Taylor,CW;Merritt,JE;PutneyJr,JW;Rubin,RP
经[3H]-肌醇预标记的大鼠腮腺电渗透性细胞在α - 1肾上腺素能、肌碱能和P物质受体激动剂刺激下合成[3H]-肌醇磷酸（ip3和IP2）。GTP的不可水解类似物（GTPγ s和GppNHp）也刺激渗透细胞形成[3H]- ip3，并增强受体激动剂的刺激作用。鸟嘌呤核苷酸的这些作用仅与GTP类似物一起发生，并且仅在通透性细胞中发生，表明其作用于细胞内。NaF刺激了[3H]- ip3的积累，这一作用并不完全归因于F-抑制（1,4,5）ip3降解的能力。这些结果表明，一种鸟嘌呤核苷酸依赖性调节蛋白将Ca2+动员受体偶联到腮腺磷脂酶C上。
Electrically permeabilized cells of rat parotid gland, prelabelled with [3H]-inositol, synthesized [3H]-inositol phosphates (IP3and IP2) when stimulated with α1-adrenergic, muscarinic-cholinergic, and substance P receptoragonists. Non-hydrolyzable analogues of GTP (GTPγS and GppNHp) also stimulated [3H]-IP3formation by permeabilized cells and they potentiated the stimulation by receptor-agonists. These effects of guanine nucleotides occurred only with GTP analogues and only in permeabilized cells indicating an intracellular site of action. NaF stimulated [3H]-IP3accumulation, an effect that was not entirely attributable to the ability of F- to inhibit (1,4,5)IP3degradation. These results suggest that a guanine nucleotide-dependent regulatory protein couples Ca2+-mobilizing receptors to phospholipase C in parotid gland.