Interactive Regulations of Dynamic Methylation and Transcriptional Responses to Recurring Environmental Stresses During Biological Invasions
Interactive Regulations of Dynamic Methylation and Transcriptional Responses to Recurring Environmental Stresses During Biological Invasions
复制标题
生物入侵过程中动态甲基化和转录反应对反复环境胁迫的交互调节
DOI：
10.3389/fmars.2021.800745
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发表时间：
2021-12-21
影响因子：
3.7
通讯作者：
Zhan, Aibin
中科院分区：
文献类型：
--
作者：
Fu, Ruiying;Huang, Xuena;Zhan, Aibin
脱氧核糖核酸甲基化和基因转录已被证明是塑料对环境压力快速反应的两种潜在机制。然而，关于DNA甲基化如何在急性和反复的环境挑战下调节基因转录以形成应激记忆，进一步促进范围扩大期间的入侵成功，目前仍不清楚。以入侵物种乔纳罗布斯塔(Ciona Robusta)为模型，研究了DNA甲基化对基因转录的调控作用，以及在入侵过程中模拟的急性和反复渗透胁迫下30个基因的应激记忆形成的作用。我们发现，攻击后68个mCpGs的甲基化水平在所有基因中呈双峰分布，但只有5个位点与相应基因的表达显著相关。这些基因参与了Ca~(2+)转运、脂类和脯氨酸代谢的生物学过程。在DNA甲基化水平上，我们发现了两个早期反应和四个迟缓反应的应激记忆位点，这些位点在功能上与参与脯氨酸生物合成、脂肪代谢以及牛磺酸和钙离子运输的基因有关。在转录水平上，3个迟反应记忆基因和5个早反应记忆基因参与离子转运、水通道调节、牛磺酸生物合成和脂质代谢。综上所述，这些发现表明DNA甲基化和基因转录应该协同工作，以促进应激记忆的形成，从而进一步提高入侵者在生物入侵过程中反复遇到环境挑战时的表现。
Deoxyribonucleic acid methylation and gene transcription have been proved as two underlying mechanisms involved in rapid plastic response to environmental stresses. However, it remains elusive on how DNA methylation regulates gene transcription under acute and recurring environmental challenges to form the stress memory, further contributing to invasion success during range expansions. Using a model invasive species Ciona robusta, we investigated the regulatory roles of DNA methylation on gene transcription and their contribution to the formation of stress memory at 30 genes under acute and recurring osmotic challenges simulated during the invasion process. We found the bimodal distribution of methylation level for the 68 mCpGs identified across all the genes after challenges, but only five sites were significantly correlated with the expression of their corresponding genes. These genes participated in the biological processes of Ca2+ transport and metabolism of lipid and proline. At the DNA methylation level, we found two early-responding and four tardy-responding sites of stress memory and these sites were functionally related to genes involved in the biosynthesis of proline, metabolism of lipid, and transport of taurine and Ca2+. At the transcriptional level, three tardy-responding and five early-responding memory genes were involved in the transport of ions, regulation of water channels, biosynthesis of taurine, and metabolism of lipid. Altogether, the findings here suggest that DNA methylation and gene transcription should work in concert to facilitate the formation of stress memory, thus further improving the performance of invaders under recurring environmental challenges during biological invasions.