Serum 5 alpha-androstane-3 alpha, 17 beta-diol increases in response to paced coital stimulation in cycling female rats.
Serum 5 alpha-androstane-3 alpha, 17 beta-diol increases in response to paced coital stimulation in cycling female rats.
复制标题
在循环雌性大鼠中，血清 5α-雄甾烷-3α、17β-二醇响应节奏性交刺激而增加。
作者：
M. Erskine
骑行的雌性大鼠自发地调节交配期间接受的宫颈-阴道刺激的量和时间。性交接触的女性起搏增加了阴道插入诱导黄体功能和缩短行为发情期的能力。在这里报道的实验中，我们检查了有节奏的交配是否会导致血清类固醇浓度的变化，这可能有助于这些过程。在实验1中，分别在交配开始后0分钟和12小时内，测定不同组别动物血清中雌二醇(E_2)、孕酮(P)、睾酮(T)、5α-二氢睾酮(DHT)和5α-雄烷-3α，17-β-二醇(3-α-雄烷二醇，3-α-二醇)的含量。在30分钟时，雌性调搏性交刺激组的3α-二醇水平显著高于非插入性刺激组或单独暴露于测试室的女性。血清T、E_2、P和DHT在任何时候在这些组之间都没有差异。在治疗后12小时内，所有组的P和3α-二醇水平均显著下降。在实验2中，比较了接受起搏刺激的雌性与接受暂时无节律性交刺激(非起搏)或仅接受坐骑刺激的雌性交配后3小时内测得的血清3α-二醇。在20min时，起搏组的血清3α-二醇浓度显著高于非起搏组和纯骑马组。在实验3中，有节奏和无节奏刺激对假孕/怀孕女性的比例没有影响。关于已知的5α减少的雄激素对性接受性的抑制作用，我们讨论了在配对的女性中选择性地增加血清3α-二醇。
The cycling female rat spontaneously regulates (paces) the amount and timing of cervical-vaginal stimulation received during mating. Female pacing of coital contacts increases the ability of vaginal intromissions to induce luteal function and to abbreviate the period of behavioral estrus. In the experiments reported here, we examined whether paced mating results in alterations in serum steroid concentrations, which might contribute to these processes. In Experiment 1, estradiol (E2), progesterone (P), testosterone (T), 5 alpha-dihydrotestosterone (DHT), and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-androstanediol, 3 alpha-diol) were measured in sera obtained from independent groups of animals between 0 min and 12 h after mating onset. Levels of 3 alpha-diol were significantly higher at 30 min in females pacing coital stimulation (Paced) than in females receiving mounts-without-intromission (Mounts-Only) or solitary exposure to the test chamber. Serum T, E2, P, and DHT did not differ between these groups at any time. P and 3 alpha-diol levels declined significantly for all groups across the 12-h post-treatment period. In Experiment 2, serum 3 alpha-diol measured over 3h after mating was compared in females receiving Paced stimulation with females receiving temporally unregulated coital stimulation (Non-Paced), or Mounts-Only. At 20 min, serum 3 alpha-diol concentrations were significantly higher in Paced than in Non-Paced and Mounts-Only females. In Experiment 3, Paced and Non-Paced stimulation did not differentially effect the proportion of females becoming pseudopregnant/pregnant. The selective increase in serum 3 alpha-diol in females that pace matings is discussed with regard to the known inhibitory effects of 5 alpha-reduced androgens on sexual receptivity.