Spatial Change of Cruciate Ligaments in Rat Embryo Knee Joint by Three-Dimensional Reconstruction.
Spatial Change of Cruciate Ligaments in Rat Embryo Knee Joint by Three-Dimensional Reconstruction.
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DOI：
10.1371/journal.pone.0131092
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Takakuwa T
中科院分区：
文献类型：
--
作者：
Zhang X;Aoyama T;Takaishi R;Higuchi S;Yamada S;Kuroki H;Takakuwa T
本研究旨在利用电子显微镜荧光图像捕捉技术(EFIC)进行三维重建，分析大鼠十字韧带的空间发育变化。对Wistar大鼠胚胎第16天至第20天的十字韧带进行了分析。使用EFIC对样本进行切片和可视化。使用Amira软件生成3D重建图。测量交叉韧带长度、附着点至股骨和胫骨的距离、十字韧带的夹角和十字韧带的交叉角。E17时十字韧带形态清晰可见。前十字韧带(ACL)和后十字韧带(PCL)的长度从E17到E19逐渐增加，到E20急剧增加。附着点与股骨和胫骨之间的距离逐渐增大。前交叉韧带角和后交叉韧带角逐渐减小。十字韧带的交叉角在三个平面上发生变化。大鼠交叉韧带三维结构的原基是从早期开始构建的，结构的发育在出生前就完成了。
This study aimed to analyze the spatial developmental changes of rat cruciate ligaments by three-dimensional (3D) reconstruction using episcopic fluorescence image capture (EFIC). Cruciate ligaments of Wister rat embryos between embryonic day (E) 16 and E20 were analyzed. Samples were sectioned and visualized using EFIC. 3D reconstructions were generated using Amira software. The length of the cruciate ligaments, distances between attachment points to femur and tibia, angles of the cruciate ligaments and the cross angle of the cruciate ligaments were measured. The shape of cruciate ligaments was clearly visible at E17. The lengths of the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) increased gradually from E17 to E19 and drastically at E20. Distances between attachment points to the femur and tibia gradually increased. The ACL angle and PCL angle gradually decreased. The cross angle of the cruciate ligaments changed in three planes. The primordium of the 3D structure of rat cruciate ligaments was constructed from the early stage, with the completion of the development of the structures occurring just before birth.
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影响因子：
19.7
作者：
Kim, Hee Kyung;Laor, Tal;Dardzinski, Bernard J.
通讯作者：
Dardzinski, Bernard J.
影响因子：
19.7
作者：
GENTILI, A;SEEGER, LL;DO, HM
通讯作者：
DO, HM
影响因子：
2.6
作者：
Weninger, Wolfgang J.;Geyer, Stefan H.
通讯作者：
Geyer, Stefan H.
DOI：
10.1007/bf00315559
发表时间：
1975-01-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
ORAHILLY, R;GARDNER, E
通讯作者：
GARDNER, E
影响因子：
4.8
作者：
Zampeli, Franceska;Ntoulia, Aikaterini;Georgoulis, Anastasios D.
通讯作者：
Georgoulis, Anastasios D.