Using In Vitro Immunomodulatory Properties of Lactic Acid Bacteria for Selection of Probiotics against Salmonella Infection in Broiler Chicks.
Using In Vitro Immunomodulatory Properties of Lactic Acid Bacteria for Selection of Probiotics against Salmonella Infection in Broiler Chicks.
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利用乳酸菌的体外免疫调节特性选择针对肉鸡沙门氏菌感染的益生菌
DOI：
10.1371/journal.pone.0147630
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Feng J;Wang L;Zhou L;Yang X;Zhao X
家禽是沙门氏菌的主要宿主。使用乳酸菌已成为控制家禽沙门氏菌的成功策略之一。本研究的目的是选择乳酸菌菌株，通过其体外免疫调节特性，为潜在的益生菌用于预防沙门氏菌感染的肉仔鸡。在101株分离的乳酸菌中，13株能在酸性（pH 2.5）和胆盐（0.1%~ 1.0%）条件下有效存活，有效抑制6种病原菌的生长，并能粘附于Caco-2细胞。然而，它们的体外免疫调节活性差异显着。最后，三种具有较高体外免疫调节特性的菌株（植物乳杆菌PZ 01、唾液乳杆菌JM 32和乳酸片球菌JH 231）和具有较低体外免疫调节活性的三种菌株（屎肠球菌JS 11，比较了唾液乳杆菌JK 22和唾液乳杆菌JM 2A 1）对沙门氏菌粘附和侵袭Caco-2的抑制作用。2细胞的体外培养和体内抗菌作用。前三株在体外抑制沙门氏菌对Caco-2细胞的粘附和侵袭，减少肠内容物、脾脏和肝脏中沙门氏菌的数量，降低血清中脂多糖诱导的TNF-α因子（LITAF）、IL-1β、IL-6和IL-12的水平，体内激发研究中增加血清中IL-10的水平比后三株更有效。这些结果表明，体外免疫调节活性可以作为额外的参数，以选择更有效的益生菌作为家禽的饲料补充剂。
Poultry is known to be a major reservoir of Salmonella. The use of lactic acid bacteria has become one of successful strategies to control Salmonella in poultry. The purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against Salmonella infection in broiler chicks. Among 101 isolated lactic acid bacteria strains, 13 strains effectively survived under acidic (pH 2.5) and bile salt (ranging from 0.1% to 1.0%) conditions, effectively inhibited growth of 6 pathogens, and adhered to Caco-2 cells. However, their in vitro immunomodulatory activities differed significantly. Finally, three strains with higher in vitro immunomodulatory properties (Lactobacillus plantarum PZ01, Lactobacillus salivarius JM32 and Pediococcus acidilactici JH231) and three strains with lower in vitro immunomodulatory activities (Enterococcus faecium JS11, Lactobacillus salivarius JK22 and Lactobacillus salivarius JM2A1) were compared for their inhibitory effects on Salmonella adhesion and invasion to Caco-2 cells in vitro and their antimicrobial effects in vivo. The former three strains inhibited Salmonella adhesion and invasion to Caco-2 cells in vitro, reduced the number of Salmonella in intestinal content, spleen and liver, reduced the levels of lipopolysaccharide-induced TNF-α factor (LITAF), IL-1β, IL-6 and IL-12 in serum and increased the level of IL-10 in serum during a challenge study in vivo more efficiently than the latter three strains. These results suggest that in vitro immunomodulatory activities could be used as additional parameters to select more effective probiotics as feed supplements for poultry.