Proteome analysis of rice uppermost internodes at the milky stage
Proteome analysis of rice uppermost internodes at the milky stage
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DOI：
10.1002/pmic.200500260
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发表时间：
2006-06
期刊：
影响因子：
3.4
通讯作者：
Pingfang Yang;Yu Liang;S. Shen;T. Kuang
中科院分区：
文献类型：
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作者：
Pingfang Yang;Yu Liang;S. Shen;T. Kuang
上节间连接着穗与下茎之间的部分，是将根的矿质营养和叶片（尤其是旗叶）的光合作用物质输送到穗的重要途径。乳白色阶段是种子成熟的第一个阶段。水稻乳浆期最上部节间对种子品质和产量至关重要。水稻（Oryza sativa L. ssp）最上部节间的总可溶性蛋白。采用蛋白质组学方法对乳白期籼稻进行分析。利用2‐DE，检测到762个可重复的蛋白斑点。其中132个富集蛋白采用MALDI‐TOF‐MS进行分析。在国家生物技术信息中心的数据库中，我们可以识别出98种蛋白质，它们代表了80种基因产物。这些蛋白质首先属于11个与能量产生相关的功能基团。参与代谢、信号传导和抗逆性的大量蛋白质积累表明，水稻最上部节间具有较高的生理和抗逆性活性。此外，我们的研究结果也将丰富水稻蛋白质组数据库。
Uppermost internodes, which connect the part between the ear and lower stem, form an important pathway transporting mineral nutrition from roots and photosynthates from leaves (especially the flag leaf) to the ear. The milky stage is the first stage of seed ripening. The uppermost internodes of rice at the milky stage are critical for seed quality and yield. Total soluble proteins of the uppermost internodes of rice (Oryza sativa L. ssp. indica) at the milky stage were analyzed using proteomic methods. Using 2‐DE, 762 reproducible protein spots were detected. Among them, 132 abundant proteins were analyzed using MALDI‐TOF‐MS. Searching in the National Center for Biotechnology Information database, we could identify 98 proteins, which represent 80 gene products. These proteins belong to 11 functional groups with energy production‐associated proteins in the first place. The large accumulation of proteins involved in metabolism, signaling, and stress resistance indicated that the uppermost internodes of rice have a high physiological and stress‐resistant activity. In addition, our results will also enrich the database of the rice proteome.