ISG20 is overexpressed in clinically relevant radioresistant oral cancer cells.
ISG20 is overexpressed in clinically relevant radioresistant oral cancer cells.
复制标题
DOI：
--
复制
发表时间：
2020-07
影响因子：
1.4
通讯作者：
H. Miyashita;Motoi Fukumoto;Y. Kuwahara;Tetsu Takahashi;Manabu Fukumoto
中科院分区：
文献类型：
--
作者：
H. Miyashita;Motoi Fukumoto;Y. Kuwahara;Tetsu Takahashi;Manabu Fukumoto
用于治疗恶性肿瘤的全球标准分割放射治疗（RT）包括2-戈伊/天的X射线照射，每周5天，持续5-7周。最近，临床相关的抗辐射（CRR）细胞首次被定义为即使在体外暴露于每日2-戈伊的X射线超过30天后仍能继续生长的细胞。为分析放射抗性癌细胞的特性，建立CRR口腔癌细胞系（CRR-OCCs），采用qRT-PCR和免疫组织化学方法检测干扰素刺激的核酸外切酶基因20（ISG 20）的表达水平。结果显示，CRR-OCC中ISG 20 mRNA及其蛋白的表达水平均高于相应的亲本细胞。我们得出结论，ISG 20在CRR-OCC中统计学上过表达。ISG 20过表达可能是CRR-OCC中放射抗性表型所必需的，靶向人癌细胞的ISG 20可能导致更有效的RT或放化疗以消除癌症。
Global standard fractionated radiotherapy (RT) for the treatment of malignancies consists of X-ray irradiation with 2-Gy/day, 5 days a week for 5-7 weeks. Recently, clinically relevant radioresistant (CRR) cells were first defined as cells that can continue to grow even after exposure to daily 2-Gy of X-rays for more than 30 days in vitro. To analyze the characteristics of radioresistant cancer cells, CRR oral cancer cells (CRR-OCCs) were established, and the expression level of interferon-stimulated exonuclease gene 20 (ISG20) was evaluated with qRT-PCR and immunohistochemical analysis. Our result revealed that the expression level of both ISG20 mRNA and its protein in CRR-OCCs were higher than those of corresponding parental cells. We concluded that ISG20 was statistically overexpressed in CRR-OCCs. ISG20 overexpression may be necessary for the radioresistant phenotype in CRR-OCCs, and targeting ISG20 of human cancer cells may lead to more efficient RT or chemoradiotherapy for eliminating cancer.