Controlling liquid-liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner
Controlling liquid-liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner
复制标题
以序列特异性方式控制 G-四链体形成 RNA 的液-液相分离
DOI：
10.1039/d2cc04366a
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Miyoshi Daisuke
中科院分区：
文献类型：
--
作者：
Tsuruta Mitsuki;Torii Takeru;Kohata Kazuki;Kawauchi Keiko;Tateishi-Karimata Hisae;Sugimoto Naoki;Miyoshi Daisuke
我们构建了一个最小的液-液相分离模型系统，仅使用G-四链体形成寡核苷酸和R-和G-丰富的寡肽形成液滴。我们发现，G-四链体结构是RNA与肽形成液滴的必要组成部分。基于这个模型系统和我们的研究结果，液滴再溶解通过从G-四链体到双链体的结构转变以序列特异性方式实现。
We constructed a minimum liquid–liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution via structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.