Eucaryotic initiation factor 4B of wheat germ binds to the translation initiation region of a messenger ribonucleic acid.
Eucaryotic initiation factor 4B of wheat germ binds to the translation initiation region of a messenger ribonucleic acid.
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小麦胚芽的真核起始因子 4B 与信使核糖核酸的翻译起始区结合。
DOI：
10.1021/bi00300a005
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
ClarkJr,JM
中科院分区：
文献类型：
--
作者：
Butler,JS;ClarkJr,JM
10-7-10-8M范围，取决于所测试的特定酶制剂。纯化的小麦胚芽eIF4A在有或无ATP存在的情况下，不能有效地与STNV RNA结合，但加入eIF4A和ATP确实能提高依赖eIF4B的STNV RNA结合效率。小麦胚芽eIF4B与含有STNV RNA 5‘端52个核苷酸的寡核苷酸(标记为1-52)结合，具有与完整STNV RNA相同的亲和力。与STNV RNA的1-44个寡核苷酸的结合亲和力较小，而与STNV RNA的1-33个包含5‘端非翻译区和启动子的寡核苷酸不存在结合亲和力
10-7—10-8 M range, depending upon the particular enzyme preparation tested. Purified wheat germ eIF4A, inthe presence or absence of ATP, does not bind STNV RNA ef-ficiently, but added eIF4A and ATP do enhance the efficiency of the eIF4B-dependent binding of STNV RNA. Wheat germ eIF4B binds the oligonucleotide containing the 5'-terminal 52 nucleotides of STNV RNA (designated 1-52) with the same affinity as intact STNV RNA. This binding affinity is less with the 1-44 oligonucleotide of STNV RNA and does not occur with the 1-33 oligonucleotide of STNV RNA that contains the 5'-terminal untranslated region and the initiator