High P-selectin expression and low CD36 occupancy on circulating platelets are strong predictors of restenosis after coronary stenting in patients with coronary artery disease
High P-selectin expression and low CD36 occupancy on circulating platelets are strong predictors of restenosis after coronary stenting in patients with coronary artery disease
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循环血小板中高 P-选择素表达和低 CD36 占用是冠状动脉疾病患者冠状动脉支架术后再狭窄的有力预测因子
作者：
K. Murasaki;M. Kawana;Satoshi Murasaki;Y. Tsurumi;K. Tanoue;N. Hagiwara;H. Kasanuki
最近的研究表明，循环血小板在冠状动脉支架植入术后早期再狭窄的发展中起重要作用。我们通过流式细胞术研究了48例接受冠状动脉支架植入术的连续患者的血小板P-选择素表达和CD 36阻断。支架术后第1天有再狭窄的患者（n = 15）P-选择素表达明显高于无再狭窄的患者（n = 28），高P-选择素水平（MFI > 6.5）患者与中低P-选择素水平患者相比，再狭窄的优势比为11.67（P < 0.001）。使用两种抗CD 36抗体OKM 5和GS 95（我们的新抗CD 36抗体）评估CD 36阻断，其结合分别指示总CD 36量和未被脂质相关配体占据的游离CD 36。两组支架植入前后OKM 5与血小板的结合相似。无再狭窄组在支架植入后第1天即可见血小板表面的CD 36被阻断，而无CD 36被阻断的患者在第1天再狭窄的优势比[GS 95结合率>0.8与支架植入前的结合率相比]为28.60（P < 0.001）。支架术后血小板P-选择素表达和CD 36的未被占据可能是支架术后再狭窄的强预测因子。
Recent studies have shown that circulating platelets play an important role in the development of restenosis early after coronary stent implantation. We investigated P-selectin expression and CD36 blockade on platelets by flow cytometry in 48 consecutive patients who underwent coronary stenting. P-selectin expression was significantly higher 1 day after stenting in patients who had restenosis (n = 15) than in those who had no restenosis (n = 28), and the odds ratio for restenosis in patients with high P-selectin levels (MFI > 6.5) was 11.67 (P < 0.001) as compared with patients who had intermediate and low P-selectin levels. CD36 blockade was assessed with the use of two anti-CD36 antibodies, OKM5 and GS95 (our new anti-CD36 antibody), the binding of which indicates total CD36 amount and free CD36 unoccupied by lipid-related ligands, respectively. Binding of OKM5 to platelets was similar before and after stenting in both groups. CD36 blockade on platelets was seen 1 day after stenting in the non-restenosis group, and the odds ratio for restenosis in patients without CD36 blockade [GS95 binding ratio >0.8 as compared with binding before stenting] on day 1 was 28.60 (P < 0.001). P-selectin expression and unoccupied CD36 on platelets shortly after stenting may be strong predictors of post-stent restenosis.
影响因子：
8.7
作者：
NICHOLSON, AC;FRIEDA, S;SILVERSTEIN, RL
通讯作者：
SILVERSTEIN, RL
影响因子：
20.3
作者：
Martha L. Aiken;M. Ginsberg;V. Byers-Ward;E. Plow
通讯作者：
Martha L. Aiken;M. Ginsberg;V. Byers-Ward;E. Plow