A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine
A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine
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DOI：
10.1016/j.dyepig.2012.05.006
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发表时间：
2012-11
影响因子：
4.5
通讯作者：
Liguo Wang;Qi Zhou;Baocun Zhu;Liang-guo Yan;Zhenmin Ma;B. Du;Xiaoling Zhang
中科院分区：
文献类型：
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作者：
Liguo Wang;Qi Zhou;Baocun Zhu;Liang-guo Yan;Zhenmin Ma;B. Du;Xiaoling Zhang
设计并合成了一种高选择性的、可见光激发的、基于共轭加成/环化序列机制的关-开荧光化学剂量计1，用于半胱氨酸（Cys）和同型半胱氨酸（Hcy）的区分检测。在模拟生理条件下，半胱氨酸和同型半胱氨酸的加入使1的溶液颜色由无色变为绿色，1可作为“肉眼”指示剂。该化学剂量计采用荧光光谱法定量检测Cys和Hcy，检测限分别为0.5 μM（Cys）和0.8 μM（Hcy）。据我们所知，1是第一个视觉和可见光激发的荧光指示剂，用于区分和同时检测Cys和Hcy。此外，1和半胱氨酸之间的反应机理进行了确认，通过ESI-MS和荧光光谱。
A highly selective, visible-light-excited and OFF–ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a “naked-eye” indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 μM (for Cys) and 0.8 μM (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra.