T lymphocytes from malnourished infants are short-lived and dysfunctional cells
T lymphocytes from malnourished infants are short-lived and dysfunctional cells
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DOI：
10.1016/j.imbio.2010.07.007
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发表时间：
2011-03-01
期刊：
影响因子：
2.8
通讯作者：
Alwasel, Saleh H.
中科院分区：
文献类型：
--
作者：
Badr, Gamal;Sayed, Douaa;Alwasel, Saleh H.
为了研究营养不良婴儿的T细胞功能分子和炎性细胞因子，并评估T细胞凋亡，招募了64名营养不良妇女的婴儿和28名健康对照婴儿。通过流式细胞术测量，营养不良婴儿的循环IL-2和IL-7水平显著降低，IL-1 β、IL-6、IL-10和TNF-α水平升高。与对照组相比，营养不良婴儿的CD 3（+）T细胞数量显著减少，凋亡T细胞增加，这与CD 3（+）T细胞上CD 95和PD-1表达上调有关。在营养不良的儿童中，还观察到Ala和STAT 5的磷酸化以及CCR 7和CXCR 4受体的表达显著减少，并且这些减少与T细胞向其配体CCL 21和CXCL 12的迁移能力显著降低相关，分别使用体外趋化性测定法测量。总之，这些数据表明，来自营养不良婴儿的淋巴细胞是短命的和功能失调的。(C)2010年Elsevier GmbH。All rights reserved.
To investigate T-cell functional molecules and inflammatory cytokines and to assess T-cell apoptosis in malnourished infants, 64 infants from undernourished women and 28 healthy control infants were recruited to the study. Malnourished infants showed a significant decrease in the levels of circulating IL-2 and IL-7 and increases in the levels of IL-1 beta, IL-6, IL-10 and TNF-alpha, as measured by flow cytometry. There was a significant reduction in the number of CD3(+) T cells and an increase in apoptotic T cells, which was associated with an up-regulation of CD95 and PD-1 expression on CD3(+) T cells in malnourished compared to control infants. Significant reductions were also observed in the phosphorylation of Ala and STAT5 and in the expression of CCR7 and CXCR4 receptors in malnourished children, and these reductions were associated with a significant reduction in T-cell migratory capacity to their ligands CCL21 and CXCL12, respectively, as measured using an in vitro chemotaxis assay. Taken together, these data suggest that lymphocytes from malnourished infants are short-lived and dysfunctional. (C) 2010 Elsevier GmbH. All rights reserved.