Altered incubation temperatures between embryonic Days 7 and 13 influence the weights and the mitochondrial respiratory and enzyme activities in breast and leg muscles of broiler embryos
Altered incubation temperatures between embryonic Days 7 and 13 influence the weights and the mitochondrial respiratory and enzyme activities in breast and leg muscles of broiler embryos
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DOI：
10.1002/mrd.22596
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发表时间：
2016-01-01
影响因子：
2.5
通讯作者：
Wicke, Michael
中科院分区：
文献类型：
--
作者：
Krischek, Carsten;Janisch, Sabine;Wicke, Michael
在胚胎发生过程中改变孵育温度对鸡胚胎生长有影响，但其潜在的分子机制尚不清楚;本研究旨在解决这些变化。在胚胎日（艾德）7和10或艾德10和13之间，在低（36.8 ℃）、对照（37.8 ℃）和高（38.8 ℃）温度下孵育肉鸡蛋，所述胚胎日涵盖胚胎肌发生的关键时期。然后在艾德10或13处理后立即解剖胚胎，以评估身体、肝脏和心脏重量，并分析乳房和腿部肌纤维的线粒体呼吸活性（MRA）。此外，还使用乳房肌肉样本来评估参与能量代谢和细胞周期进程的酶的活性。与在36.8 ℃下孵育的艾德-10胚胎相比，在38.8 ℃下孵育的艾德-10胚胎的重量（体重、肝脏和心脏）、MRA以及乳酸脱氢酶和细胞色素氧化酶的活性升高。同样，与36.8 ℃的胚胎相比，在38.8 ℃孵育的艾德-13胚胎显示出体重、MRA和糖原磷酸化酶、磷酸果糖激酶和细胞色素氧化酶的活性升高。然而，在正常温度（37.8摄氏度）下孵育的胚胎与在38.8摄氏度和36.8摄氏度下孵育的胚胎表现出不同的差异。细胞周期酶活性不受不同温度处理的影响。因此，在胚胎肉鸡肌肉发生过程中，孵育温度的升高或降低会导致胚胎活动、肌肉能量代谢和活动依赖性肌肉生长的改变。摩尔Reprod. Dev. 83：71-78，2016. (c)2015 Wiley Periodicals，Inc.
Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8 degrees C), control (37.8 degrees C), and high (38.8 degrees C) temperatures between Embryonic Day (ED) 7 and 10 or ED 10 and 13, which cover critical periods of embryonic myogenesis. The embryos were then dissected immediately after treatment on ED 10 or 13 to assess body, liver, and heart weights as well as to analyze breast and leg muscle fibers for their mitochondrial respiratory activity (MRA). Breast muscle samples were additionally used to evaluate the activity of enzymes involved in energy metabolism and cell-cycle progression. ED-10 embryos incubated at 38.8 degrees C showed elevated weights (body, liver, and heart), MRA, and activities of lactate dehydrogenase and cytochrome oxidase compared to the ED-10 embryos incubated at 36.8 degrees C. Similarly, the ED-13 embryos incubated at 38.8 degrees C showed elevated body weight, MRA, and activities of glycogen phosphorylase, phosphofructokinase, and cytochrome oxidase compared to their 36.8 degrees C counterparts. Embryos incubated at the normal temperature (37.8 degrees C), however, showed variable differences from those incubated at 38.8 degrees C versus 36.8 degrees C. Cell-cycle enzyme activities were not impacted by the different temperature treatments. Thus, an increase or decrease in the incubation temperature during embryonic broiler myogenesis results in altered embryo activity, muscle energy metabolism, and activity-dependent muscle growth. Mol. Reprod. Dev. 83: 71-78, 2016. (c) 2015 Wiley Periodicals, Inc.