Transcriptional profiling in rats and an ex vivo analysis implicate novel beneficial function of egg shell membrane in liver fibrosis
Transcriptional profiling in rats and an ex vivo analysis implicate novel beneficial function of egg shell membrane in liver fibrosis
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大鼠转录谱和离体分析表明蛋壳膜在肝纤维化中具有新的有益功能
DOI：
10.1016/j.jff.2013.07.003
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发表时间：
2013
影响因子：
5.6
通讯作者：
Hisanori Kato
中科院分区：
文献类型：
--
作者：
Huijuan Jia;Kenji Saito;Wanping Aw;Shoko Takahashi;Manaka Hanate;Yukio Hasebe;Hisanori Kato
蛋壳膜（ESM）是鸡蛋加工过程中产生的一种天然安全的食品副产品，但有关ESM作为食品成分的作用的信息很少。采用基因芯片技术研究膳食ESM对大鼠肝脏基因表达的影响。ESM处理14天后，发现平滑肌-α-肌动蛋白（肝星状细胞（HSC）活化的标志物）和整合素β-样1、核心蛋白聚糖、asporin、lumican和胶原1型α 1（细胞外基质（ECM）的组分并参与HSC活化和纤维化的上调）的表达显著下调。随后，从给予ESM饮食的大鼠获得的血清也抑制了人肝癌C3 A细胞中COL 1A 1和COL 1A 2的表达。Ourin vivoandex vivo研究结果表明，这些基因改变可能有助于ESM的有益作用，部分通过下调c-jun和c-fos信号转导，从而钝化HSC活化，并最终防止肝纤维化。这些结果不仅提供了关于无害环境管理的功能和营养可用性的新信息，而且可能有助于环境保护领域。
Egg shell membrane (ESM) is a natural and safe food by-product from egg processing whilst there is little information about the role of ESM as a food component. Effects of dietary ESM on gene expression in rat liver were investigated through DNA microarray comprehensive analysis. The expression of smooth muscle-α-actin, which is a marker of hepatic stellate cells (HSCs) activation, and integrin beta-like 1, decorin, asporin, lumican and collagen type 1 alpha 1, which are components of extracellular matrix (ECM) and involved in the up-regulation of HSCs activation and fibrosis, was found to be significantly down-regulated after 14 days of ESM treatment. Subsequently, serum obtained from rats given ESM diet also suppressed the expression ofCOL1A1andCOL1A2in human hepatoma C3A cells. Ourin vivoandex vivofindings demonstrate that these gene alterations may contribute to the beneficial effect of ESM partially through down-regulation of c-jun and c-fos signal transduction thereby blunting HSCs activation and eventually preventing liver fibrosis. These outcomes not only provide novel information about the functional and nutritional availability of ESM, but also might contribute to the field of environmental protection.