MaJAZ1 Attenuates the MaLBD5-Mediated Transcriptional Activation of Jasmonate Biosynthesis Gene MaAOC2 in Regulating Cold Tolerance of Banana Fruit
MaJAZ1 Attenuates the MaLBD5-Mediated Transcriptional Activation of Jasmonate Biosynthesis Gene MaAOC2 in Regulating Cold Tolerance of Banana Fruit
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DOI：
10.1021/acs.jafc.5b05005
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发表时间：
2016-02-03
影响因子：
6.1
通讯作者：
Lug, Wang-jin
中科院分区：
文献类型：
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作者：
Ba, Liang-jie;Kuang, Jian-fei;Lug, Wang-jin
茉莉酸甲酯（MeJA）处理可以有效地减轻包括香蕉在内的许多水果的冷害，但其机制尚不清楚。本研究从香蕉果实中克隆了一个LBD基因，命名为MaLBDS。表达分析表明，低温和MeJA处理诱导MaLBDS的积累。亚细胞定位和转录激活实验表明，MaLBDS定位于细胞核，具有转录激活活性。蛋白质相互作用分析表明，MaLBDS物理相互作用与MaJAZ1，茉莉酸信号的潜在阻遏物。此外，瞬时表达试验表明，MaLBDS反式激活茉莉酸生物合成基因，称为MaAOC2，这也是由冷和MeJA诱导。更有趣的是，MaJAZ1减弱了MaLBDS介导的MaAOC2的反式激活。这些结果表明，MaLBDS和MaJAZ 1可能起拮抗作用的关系，MeJA诱导的香蕉果实的耐冷性，至少部分通过影响茉莉酸的生物合成。总的来说，我们的研究结果扩大了知识的转录调控网络的MeJA介导的香蕉果实的耐冷性。
Previous studies indicated that methyl jasmonate (MeJA) treatment could effectively reduce the chilling injury of many fruits, including banana, but the underlying mechanism is poorly understood. In this study, one lateral organ boundaries (LOB) domain (LBD) gene, designated as MaLBDS, was isolated and characterized from banana fruit. Expression analysis revealed that accumulation of MaLBDS was induced by cold temperature and MeJA treatment. Subcellular localization and transactivation assays showed that MaLBDS was localized to the nucleus and possessed transcriptional activation activity. Protein protein interaction analysis demonstrated that MaLBDS physically interacted with MaJAZ1, a potential repressor of jasmonate signaling. Furthermore, transient expression assays indicated that MaLBDS transactivated a jasmonate biosynthesis gene, termed MaAOC2, which was also induced by cold and MeJA. More interestingly, MaJAZ1 attenuated the MaLBDS-mediated transactivation of MaAOC2. These results suggest that MaLBDS and MaJAZ1 might act antagonistically in relation to MeJA-induced cold tolerance of banana fruit, at least partially via affecting jasmonate biosynthesis. Collectively, our findings expand the knowledge of the transcriptional regulatory network of MeJA-mediated cold tolerance of banana fruit.