Activation status of T and NK cells in the endometrium throughout menstrual cycle and normal and abnormal early pregnancy
Activation status of T and NK cells in the endometrium throughout menstrual cycle and normal and abnormal early pregnancy
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DOI：
10.1016/0198-8859(96)00120-6
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发表时间：
1996-09-01
期刊：
影响因子：
2.7
通讯作者：
Huang, SC
中科院分区：
文献类型：
--
作者：
Ho, HN;Chao, KH;Huang, SC
通过流式细胞术对整个月经周期和妊娠早期的各个阶段的子宫内膜淋巴细胞进行研究，以检查不同的淋巴细胞亚群以及 T 细胞和 NK 细胞激活标记物的表达。妊娠后，蜕膜中CD8(+)CD3(+)淋巴细胞减少。在子宫内膜和蜕膜中，更多的T细胞表达CD69、CD71。 HLA-DR和CD38抗原高于外周血。妊娠后CD71(+)CD3(+)淋巴细胞进一步增加。异位妊娠子宫内膜和蜕膜中CD25(+)CD3(+)淋巴细胞明显减少，而正常妊娠蜕膜中无明显变化。这些发现表明，T细胞在妊娠早期出现区域性激活，这可能是胎儿抗原刺激的结果。 NK细胞是蜕膜中最丰富的细胞类型，表达CD16(-) CD56(+)表型。和CD57(-)CD56(+)。尽管两组的 NK 亚群总数相似，但无胚胎妊娠中活化的蜕膜 NK 细胞的比例比正常妊娠中增加更多。这可能。导致无胚胎妊娠时 NK 细胞毒性增加。总之，正常妊娠早期蜕膜中T细胞被激活，但NK细胞毒性降低。这对于控制滋养层生长和胎盘发育可能很重要。
Endometrial lymphocytes were studied at all stages throughout the menstrual cycle and early pregnancy by flow cytometry to examine different lymphocyte subpopulations and the expression of the T- and NK-cell activation markers. After pregnancy, CD8(+)CD3(+) lymphocytes were decreased in the decidua. In both endometrium and decidua, more T cells expressed CD69, CD71. HLA-DR, and CD38 antigens than in peripheral blood. After pregnancy, CD71(+)CD3(+) lymphocytes were further increased. CD25(+)CD3(+) lymphocytes decreased significantly in the endometrium and decidua of ectopic pregnancies, but not in the decidua of normal pregnancies. These findings indicate that T cells are regionally activated in the first trimester, and it may be the result of the stimulation by fetal antigens. NK cells were the most abundant cell type in the decidua, which expressed the phenotype CD16(-) CD56(+). and CD57(-)CD56(+). The proportion of activated decidual NK cells was increased in anembryonic pregnancies more than in normal pregnancies, although the total NK subpopulation was similar in both groups. This might. result in increased NK cytotoxicity in anembryonic pregnancies. In conclusion, T cells are activated, but NK cytotoxicity is decreased in the decidua of early normal pregnancies. This might be important in the control of trophoblast growth and placental development.