Mesenchymal stromal cell and osteoblast responses to oxidized titaniumsurfaces pre-treated with λ=808 nm GaAlAs diode laser or chlorhexidine: in vitro study
Mesenchymal stromal cell and osteoblast responses to oxidized titaniumsurfaces pre-treated with λ=808 nm GaAlAs diode laser or chlorhexidine: in vitro study
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DOI：
10.1007/s10103-017-2243-5
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发表时间：
2017-08-01
影响因子：
2.1
通讯作者：
Sassoli, Chiara
中科院分区：
文献类型：
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作者：
Chellini, Flaminia;Giannelli, Marco;Sassoli, Chiara
种植体周围炎治疗后保持种植体生物相容性是口腔医学实践中的一个关键问题，与种植体再骨整合密切相关。我们的目的是评估成骨细胞样 Saos2 细胞和成人骨髓间充质基质细胞 (MSC) 对氧化钛表面 (TiUnite (R), TiU) 的反应，这些表面用 808 +/- 10 nm GaAlAs 二极管激光器以非接触模式、连续（2 W、400 J/cm(2)；CW）或脉冲（20 kHz、7 mu s、 0.44 W，88 J/cm(2)；PW）波，先前已被证明具有很强的杀菌作用，并被建议作为种植体周围炎的可选治疗方法。还评估了用二葡萄糖酸氯己定 (CHX) 预处理的 TiU 表面的生物相容性。特别是，为了模拟体内方法，TiU 表面用 CHX 进行预处理（0.2%，5 分钟）； CHX并冲洗；和CHX，冲洗并风干。在一些实验中，细胞在暴露于 CHX 之前在未经处理的 TiU 上培养。进行细胞活力（MTS 测定）、增殖（EdU 掺入测定；Ki67 共聚焦免疫荧光分析）、粘附（肌动蛋白细胞骨架组织的形态学分析）和成骨分化（骨桥蛋白共聚焦免疫荧光分析；矿化骨样结节形成）分析。 CHX 在所有实验条件下均产生细胞毒性。二极管激光照射保留了 TiU 表面的生物相容性。值得注意的是，激光治疗似乎甚至可以改善 TiU 表面已知的骨传导特性。在体外实验的限制内，本研究有助于提供额外的实验基础，以支持在指定的照射设置下使用 808 +/- 10 nm GaAlAs 二极管激光器治疗种植体周围炎并阻止 CHX 的使用。
Preservation of implant biocompatibility following peri-implantitis treatments is a crucial issue in odontostomatological practice, being closely linked to implant re-osseointegration. Our aim was to assess the responses of osteoblast-like Saos2 cells and adult human bone marrow-mesenchymal stromal cells (MSCs) to oxidized titanium surfaces (TiUnite (R), TiU) pre-treated with a 808 +/- 10 nm GaAlAs diode laser operating in non-contact mode, in continuous (2 W, 400 J/cm(2); CW) or pulsed (20 kHz, 7 mu s, 0.44 W, 88 J/cm(2); PW) wave, previously demonstrated to have a strong bactericidal effect and proposed as optional treatment for peri-implantitis. The biocompatibility of TiU surfaces pre-treated with chlorhexidine digluconate (CHX) was also evaluated. In particular, in order to mimic the in vivo approach, TiU surfaces were pre-treated with CHX (0.2%, 5 min); CHX and rinse; and CHX, rinse and air drying. In some experiments, the cells were cultured on untreated TiU before being exposed to CHX. Cell viability (MTS assay), proliferation (EdU incorporation assay; Ki67 confocal immunofluorescence analysis), adhesion (morphological analysis of actin cytoskeleton organization), and osteogenic differentiation (osteopontin confocal immunofluorescence analysis; mineralized bone-like nodule formation) analyses were performed. CHX resulted cytotoxic in all experimental conditions. Diode laser irradiation preserved TiU surface biocompatibility. Notably, laser treatment appeared even to improve the known osteoconductive properties of TiU surfaces. Within the limitations of an in vitro experimentation, this study contributes to provide additional experimental basis to support the potential use of 808 +/- 10 nm GaAlAs diode laser at the indicated irradiation setting, in the treatment of peri-implantitis and to discourage the use of CHX.