DNA sequence detection using selective fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles
DNA sequence detection using selective fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles
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DOI：
10.1021/ac049173n
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发表时间：
2004-09-15
影响因子：
7.4
通讯作者：
Rothberg, LJ
中科院分区：
文献类型：
--
作者：
Li, HX;Rothberg, LJ
对特定DNA序列的简单，快速，经济的检测对于病原体检测和生物医学研究至关重要。我们已经根据DNA的静电特性设计了一种新型的荧光测定，用于DNA杂交。我们利用创造条件的能力，即单链DNA吸附在带负电荷的金纳米颗粒上而双链DNA没有。当染料标签的探针序列与金纳米颗粒混合时，除非与分析物的成分杂交，否则将其荧光有效淬灭。使用市售材料在几分钟内检测到无标记目标的亚femtomole量。很容易检测到DNA和单碱基不匹配的复杂混合物中的目标序列。
Simple, fast, economical, and sensitive detection of specific DNA sequences is crucial to pathogen detection and biomedical research. We have designed a novel fluorescent assay for DNA hybridization based on the electrostatic properties of DNA. We exploit the ability to create conditions where single-stranded DNA adsorbs on negatively charged gold nanoparticles while double-stranded DNA does not. Dye-tagged probe sequences have their fluorescence efficiently quenched when they are mixed with gold nanoparticles unless they hybridize with components of the analyte. Subfemtomole amounts of untagged target are detected in minutes using commercially available materials. Target sequences in complex mixtures of DNA and single-base mismatches in DNA sequences are easily detected.