Determination of nevirapine, an HIV-1 non-nucleoside reverse transcriptase inhibitor, in human plasma by reversed-phase high-performance liquid chromatography
Determination of nevirapine, an HIV-1 non-nucleoside reverse transcriptase inhibitor, in human plasma by reversed-phase high-performance liquid chromatography
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DOI：
10.1016/s0378-4347(00)00231-0
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发表时间：
2000-07-07
期刊：
影响因子：
--
通讯作者：
Hekster, YA
中科院分区：
文献类型：
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作者：
Hollanders, RMF;Kolmer, EWJV;Hekster, YA
建立了一种灵敏、快速的高效液相色谱法测定人血浆中HIV-1非核苷类逆转录酶抑制剂奈韦拉平的浓度。样品预处理包括用高氯酸沉淀蛋白质。采用Hypersil ODS柱，室温，紫外检测波长280 nm。移动的流动相含有乙腈和60 mM磷酸盐缓冲液pH 4.5（30：70，v/v）。使用150 μ l血浆，该方法的检测限为0.05 mg/l。定量下限和上限分别为0.1 mg/l和10 mg/l。奈韦拉平的平均回收率为101.8%，变异系数为4.6%。平均批间精密度为2.4%，平均批内精密度为2.9%，平均准确度为97%。(C)2000 Elsevier Science BN。All rights reserved.
A sensitive and rapid high-performance Liquid chromatography method has been developed to measure the levels of the HIV-1 non-nucleoside reverse transcriptase inhibitor nevirapine in human plasma. The sample pre-treatment consists of a protein precipitation with perchloric acid. A Hypersil ODS column is used at ambient temperature and a wavelength of 280 nm is used for ultraviolet detection. The mobile phase contains acetonitrile and a 60 mM phosphate buffer pH 4.5 (30:70, v/v). The detection limit of the method is 0.05 mg/l using 150 mu l of plasma. The lower and upper limit of quantitation are 0.1 mg/l and 10 mg/l, respectively. The average recovery of nevirapine is 101.8% with a variation of 4.6%. The average inter-assay precision is 2.4%, the average intra-assay precision 2.9% and the average accuracy 97%. (C) 2000 Elsevier Science BN. All rights reserved.