High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes
High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes
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DOI：
10.1073/pnas.0601211103
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发表时间：
2006-03-28
影响因子：
11.1
通讯作者：
Adhya, S
中科院分区：
文献类型：
--
作者：
Edgar, R;McKinstry, M;Adhya, S
随着当前对耐药细菌和生物防御的关注，快速识别感染性细菌变得重要。涉及病原菌的分离和扩增的传统技术是耗时的。我们报告了一种快速而简单的方法，该方法结合了体内生物素化的工程化宿主特异性噬菌体和噬菌体与链霉亲和素包被的量子点的缀合。该方法提供了在实验样品中每毫升少至10个细菌细胞的特异性检测，在1 In中的背景信号放大约100倍。我们相信，该方法可以应用于对特定细菌敏感的任何细菌，并且特别适用于检测生长缓慢的细菌菌株，例如，分枝杆菌，或者是高度传染性的，例如，炭疽杆菌。讨论了在单个样品中同时检测不同细菌种类的可能性和在噬菌体生物学研究中的应用。
With current concerns of antibiotic-resistant bacteria and biodefense, it has become important to rapidly identify infectious bacteria. Traditional technologies involving isolation and amplification of the pathogenic bacteria are time-consuming. We report a rapid and simple method that combines in vivo biotinylation of engineered host-specific bacteriophage and conjugation of the phage to streptavidin-coated quantum dots. The method provides specific detection of as few as 10 bacterial cells per milliliter in experimental samples, with an approximate to 100-fold amplification of the signal over background in 1 In. We believe that the method can be applied to any bacteria susceptible to specific phages and would be particularly useful for detection of bacterial strains that are slow growing, e.g., Mycobacterium, or are highly infectious, e.g., Bacillus anthracis. The potential for simultaneous detection of different bacterial species in a single sample and applications in the study of phage biology are discussed.