Evaluation of immune responses induced by SAG1 and MIC3 vaccine cocktails against Toxoplasma gondii
Evaluation of immune responses induced by SAG1 and MIC3 vaccine cocktails against Toxoplasma gondii
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SAG1 和 MIC3 混合疫苗针对弓形虫诱导的免疫反应评估
DOI：
10.1016/j.vetpar.2011.12.007
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发表时间：
2012-06-08
影响因子：
2.6
通讯作者：
Zhao, Junlong
中科院分区：
文献类型：
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作者：
Fang, Rui;Feng, Hanli;Zhao, Junlong
本研究的目的是评价SAG1和MiC3疫苗鸡尾酒对BALB/c小鼠的免疫应答。将96只BALB/c小鼠随机分为8组，每组10只，分别为3个质粒DNA疫苗组(pcDNAMiC3、pcDNASAG1、pcDNAMiC3+pcDNASAG1)、3个重组伪型杆状病毒疫苗组(BV-G-MiC3、BV-G-SAG1、BV-G-SAG1+BV-G-MiC3)和2个对照组(PBS和BV-G-EGFP)。所有组均每隔三周肌肉注射免疫两次。观察免疫后小鼠抗弓形虫裂解抗原(TLA)抗体的产生、淋巴细胞增殖、干扰素-γ、白介素4和白介素10水平以及小鼠的存活时间。结果表明，MiC3疫苗和SAG1疫苗免疫BALB/c小鼠，均能刺激细胞免疫和体液免疫，并产生抗T。弓形虫TLA抗体。PcDNA-MiC3+pcDNA-SAG1或BV-G-SAG1+BV-G-MiC3混合疫苗诱导的免疫原性显著高于单基因疫苗(P
The aim of this study was to evaluate the immune responses of a SAG1 and MIC3 vaccine cocktail in BALB/c mice. Ninety-six BALB/c mice were randomly divided into eight groups, including three plasmid DNA vaccine groups (pcDNA-MIC3, pcDNA-SAG1, pcDNA-MIC3 + pcDNA-SAG1), three recombinant pseudotype baculovirus vaccine groups (BV-G-MIC3, BV-G-SAG1, BV-G-SAG1 + BV-G-MIC3) and two control groups (PBS and BV-G-EGFP). All groups were immunized intramuscularly twice at three-week intervals. The production of anti-Toxoplasma gondii lysate antigen (TLA) antibodies, lymphoproliferation, levels of IFN-gamma, IL-4 and IL-10 and the survival time were monitored after vaccination. The results showed that immunization of BALB/c mice with MIC3 and SAG1 vaccines stimulated both the cellular and humoral immune responses with the production of anti-T. gondii TLA antibodies. The vaccine cocktails of pcDNA-MIC3 + pcDNA-SAG1 or BV-G-SAG1 + BV-G-MIC3 induced significantly higher immunogenicity than a single-gene vaccine (P