Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy.
Curcumin-loaded redox response of self-assembled micelles for enhanced antitumor and anti-inflammation efficacy.
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自组装胶束负载姜黄素的氧化还原反应增强抗肿瘤和抗炎功效
DOI：
10.2147/ijn.s123190
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发表时间：
2017
影响因子：
8
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Zhao S;Ma L;Cao C;Yu Q;Chen L;Liu J
目前，炎症在肿瘤生长中起着至关重要的作用，同时，使用纳米载体的化疗药物已被认为是一种有前途的癌症治疗策略。本研究以单甲氧基聚乙二醇-壳聚糖-S-S-十六烷基（C16-SS-CS-mPEG）为原料，制备了新型氧化还原响应性胶束。这些胶束能够携带和递送药物进入肿瘤细胞。为了作为对照，以与用于产生不具有氧化还原响应性二硫键的C16-CC-CS-mPEG的方式类似的方式开发单甲氧基-聚（乙二醇）-壳聚糖-C-C-十六烷基（C16-CC-CS-mPEG）。两种胶束的细胞摄取机制进行了测定。进一步证实了胶束在MCF-7细胞中的有效胞内药物释放。结果表明，姜黄素（Cur）在还原剂作用下可迅速形成C16-SS-CS-mPEG@ Cur胶束，并有效地促进细胞内蓄积。细胞毒性试验表明C16-SS-CS-mPEG@Cur对MCF-7细胞具有良好的细胞毒性。抗炎实验结果表明，C16-SS-CS-mPEG@Cur处理显著下调肿瘤坏死因子（TNF-α）的表达，对肿瘤微环境具有良好的抗炎作用。最重要的是，C16-SS-CS-mPEG@Cur的体内抗肿瘤作用显示出令人满意的治疗效果。因此，C16-SS-CS-mPEG@Cur胶束可用于肿瘤治疗。
At present, it has become evident that inflammation plays a critical role in tumor growth; meanwhile, chemotherapeutic agents using nanocarriers have been suggested as a promising strategy in cancer treatment. In this study, novel redox-responsive micelles were prepared from monomethoxy-poly(ethylene glycol)-chitosan-S-S-hexadecyl (C16-SS-CS-mPEG). These micelles were able to carry and deliver drugs into tumor cells. To serve as a control, monomethoxy-poly(ethylene glycol)-chitosan-C-C-hexadecyl (C16-CC-CS-mPEG) was developed in a similar fashion to that used to yield C16-CC-CS-mPEG without a redox-responsive disulfide bond. The cellular uptake mechanisms of both micelles were determined. The efficient intracellular drug release from micelles in MCF-7 cells was further confirmed. Results indicated that curcumin (Cur) could rapidly form C16-SS-CS-mPEG@ Cur micelles when exposed to reducing agents and efficaciously enhance intracellular accumulation. The cytotoxicity assay demonstrated that C16-SS-CS-mPEG@Cur exhibited satisfactory cytotoxicity against MCF-7 cells. Anti-inflammation assay results indicated that C16-SS-CS-mPEG@Cur treatment significantly downregulated tumor necrosis factor (TNF-α) expression and showed good anti-inflammatory effects in tumor microenvironment. Most importantly, antitumor effects in vivo showed satisfactory therapeutic effects with C16-SS-CS-mPEG@Cur. Hence, C16-SS-CS-mPEG@Cur micelles can be useful in tumor therapy.