Localization of type I human skin collagenase in developing embryonic and fetal skin.
Localization of type I human skin collagenase in developing embryonic and fetal skin.
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I 型人类皮肤胶原酶在发育中的胚胎和胎儿皮肤中的定位。
DOI：
10.1111/1523-1747.ep12384127
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Smith,LT
中科院分区：
文献类型：
--
作者：
McGowan,KA;Bauer,EA;Smith,LT
I型人皮肤胶原酶（HSC-1）定位于发育中的胚胎和胎儿皮肤，估计胎龄为6至20周，使用抗原特异性，亲和纯化，多克隆抗血清HSC-1和抗生物素蛋白-生物素碱性磷酸酶程序。双免疫标记与单克隆抗体因子VIII相关的抗原，IV型胶原蛋白，和68千道尔顿的神经丝亚基进行使用直接peroxidaseprocedure.By 8周估计胎龄，HSC-1本地化的perididendum，基底细胞表皮角质形成细胞，真皮成纤维细胞，和周围的细胞外基质。在12周的估计胎龄，HSC-1免疫标记显示与表皮和真皮的持续关联。真皮和皮下血管及周围细胞外基质HSC-1标记阳性。HSC-1染色也发现周围发育的神经和与真皮成纤维细胞。在毛囊的发育过程中，HSC-1存在于前胚、胚、毛栓和球状毛栓的角质形成细胞中。HSC-1免疫反应也被发现与毛管，隆起，真皮乳头，但缺席胎儿皮脂腺。这些数据表明HSC-1与毛囊间表皮、真皮胶原基质、血管生成过程、神经发育和毛囊形态发生的发育相关。
Type I human skin collagenase (HSC-1) was localized in developing embryonic and fetal skin ranging from 6 to 20 weeks estimated gestational age using an antigen-specific, affinity-purified, polyclonal antiserum to HSC-1 and an avidin-biotin alkaline phosphatase procedure. Double immunolabeling with monoclonal antibodies for Factor VIII-related antigen, type IV collagen, and the 68-kilodalton neurofilament subunit was performed using a direct peroxidase procedure.By 8 weeks estimated gestational age, HSC-1 localized to the periderm, the basal cell epidermal keratinocytes, dermal fibroblasts, and surrounding extracellular matrix. At 12 weeks estimated gestational age, HSC-1 immunolabeling showed a continued association with the epidermis and dermis. Dermal and subcutaneous blood vessels and the surrounding extracellular matrix were positive for HSC-1 labeling. HSC-1 staining was also found around developing nerves and in association with dermal fibroblasts. In the developing hair follicle, HSC-1 was present in keratinocytes of the pre-germ, germ, hair peg, and bulbous hair peg. HSC-1 immunoreactivity was also found in association with the hair canal, the bulge, and the dermal papillae, but was absent from the fetal sebaceous gland. These data demonstrate the association of HSC-1 with the development of interfollicular epidermis, the dermal collagenous matrix, the process of angiogenesis, the development of nerves, and hair follicle morphogenesis.