A simple method for the separate measurement of transcobalamins I, II, and III: normal ranges in serum and plasma in men and women.
A simple method for the separate measurement of transcobalamins I, II, and III: normal ranges in serum and plasma in men and women.
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单独测量转钴胺素 I、II 和 III 的简单方法：男性和女性血清和血浆中的正常范围。
DOI：
10.5555/uri:pii:0022214377900506
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
Victor Herbert
中科院分区：
文献类型：
--
作者：
Elizabeth Jacob;Elizabeth Jacob;Kit;Kit;Victor Herbert;Victor Herbert
描述了一种简单的方法，用于单独测量三种主要循环维生素B12结合剂的不饱和结合能力，transcobaltamine（TC）I，II和III。该方法包括通过Quso G32将TC I和III与TC II分批分离，然后使用二乙基氨基乙基将TC I与TC II分批分离。本研究提供了第一个完整的比较不饱和TC I，II和III的血清与氟化血浆的一组正常男性与女性。有一贯较高的值在血清中，主要是由于在体外增加TC III，防止氟化钠。结果进行了比较，其中一些参数的值由其他人使用不同的方法，提供了一个标准，评价结果的意义，通过各种方法。
A simple method for the separate measurement of the unsaturated binding capacity of the three main circulating vitamin B12 binders, transcobalamins (TCs) I, II, and III, is described. The method involves batch separation of TC I and III from TC II by means of Quso G32, followed by batch separation of TC I from TC II with the use of diethylaminoethyl. The present study provides the first complete comparison of unsaturated TC I, II, and III in serum versus fluoridinated plasma of a single group of normal men versus women. There are consistently higher values in serum, largely due to an in vitro increment of TC III, prevented by NaF. The results are compared with values for some of these parameters obtained by others with the use of different methods, providing a standard for evaluating significance of results by various methods.