Tissue distribution of immunoreactive mouse extracellular superoxide dismutase
Tissue distribution of immunoreactive mouse extracellular superoxide dismutase
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DOI：
10.1152/ajpcell.1998.275.3.c840
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发表时间：
1998-09-01
影响因子：
5.5
通讯作者：
Ohno, H
中科院分区：
文献类型：
--
作者：
Ookawara, T;Imazeki, N;Ohno, H
本文研究了小鼠16种组织中细胞外超氧化物歧化酶（EC-SOD）的蛋白质含量和mRNA表达。我们建立了双抗体夹心ELISA使用亲和纯化的IgG对天然小鼠EC-SOD。EC-SOD可在所有检查的组织中检测到（肺、肾、睾丸、棕色脂肪、肝、肾上腺、胰腺、结肠、白色脂肪、胸腺、胃、脾、心脏、骨骼肌、回肠和脑，以μ g/g湿组织测量的含量降序排列）。肺组织EC-SOD活性明显高于其它组织。有趣的是，白色脂肪中EC-SOD的含量很高，以微克/毫克蛋白质计，这与肺中的含量相对应。肾脏EC-SOD mRNA表达最强。在肺、白色脂肪、肾上腺、棕色脂肪和睾丸中观察到相对较强的mRNA表达。心脏和大脑仅显示出微弱的信号，并且在消化器官和骨骼肌中均未检测到这种表达。免疫组化显示EC-SOD主要定位于结缔组织和血管壁。在肾皮质小管细胞中观察到细胞质中的深染色。这些结果表明，EC-SOD是全身分布在小鼠和这种酶的生理重要性可能是一种代偿性适应氧化应激，特别是在肺和肾。
Protein content and mRNA expression of extracellular superoxide dismutase (EC-SOD) were investigated in 16 mouse tissues. We developed a double-antibody sandwich ELISA using the affinity-purified IgG against native mouse EC-SOD. EC-SOD could be detected in all of the tissues examined (lung, kidney, testis, brown fat, liver, adrenal gland, pancreas, colon, white fat, thymus, stomach, spleen, heart, skeletal muscle, ileum, and brain, in decreasing order of content measured as mu g/g wet tissue). Lung showed a markedly higher value of EC-SOD than other tissues. Interestingly, white fat had a high content of EC-SOD in terms of micrograms per milligram protein, which corresponded to that of lung. Kidney showed the strongest expression of EC-SOD mRNA. Relatively strong expression of the mRNA was observed in lung, white fat, adrenal gland, brown fat, and testis. Heart and brain showed only weak signals, and no such expression could be detected in either digestive organs or skeletal muscle. Immunohistochemically, EC-SOD was localized mainly to connective tissues and vascular walls in the tissues examined. Deep staining in the cytosol was observed in the cortical tubular cells of kidney. These results suggest that EC-SOD is distributed systemically in mice and that the physiological importance of this enzyme may be a compensatory adaptation to oxidative stress, particularly in lung and kidney.