Protease-activated receptor-2 (PAR-2) in the rat gastric mucosa: immunolocalization and facilitation of pepsin/pepsinogen secretion
Protease-activated receptor-2 (PAR-2) in the rat gastric mucosa: immunolocalization and facilitation of pepsin/pepsinogen secretion
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DOI：
10.1038/sj.bjp.0704562
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发表时间：
2002-03-01
影响因子：
7.3
通讯作者：
Kawabata, A
中科院分区：
文献类型：
--
作者：
Kawao, N;Sakaguchi, Y;Kawabata, A
1蛋白酶活化受体2 （PAR-2）激动剂触发胃中神经介导的粘液分泌，并伴有粘膜细胞保护。本研究将PAR-2免疫定位于大鼠胃粘膜，观察PAR-2是否能调节胃蛋白酶/胃蛋白酶原的分泌胃粘膜深部有丰富的par -2样免疫反应性，特别是在主细胞中以0.3-1 μ mol kg（-1）的剂量反复静脉注射4次，PAR-2激动剂SLIGRL-NH2可显著促进胃蛋白酶的分泌，但对照肽LSIGRL-NH2无显著作用par -2介导的胃蛋白酶分泌对奥美拉唑、n - g -硝基- l -精氨酸甲酯（n - g -硝基- L-NAME）或阿托品有抗性，对辣椒素消融感觉神经元也有抗性因此我们的研究提供了新的证据，证明PAR-2定位于粘膜主细胞并促进大鼠胃蛋白酶的分泌。很可能是通过直接机制。英国药理学杂志（2002）。
1 Agonists of protease-activated receptor-2 (PAR-2) trigger neurally mediated mucus secretion accompanied by mucosal cytoprotection in the stomach. The present study immunolocalized PAR-2 in the rat gastric mucosa and examined if PAR-2 could modulate pepsin/pepsinogen secretion in rats.2 PAR-2-like immunoreactivity was abundant in the deep regions of gastric mucosa, especially in chief cells.3 The PAR-2 agonist SLIGRL-NH2, but not the control peptide LSIGRL-NH2 adininistered i.v. repeatedly at 0.3-1 mumol kg(-1), four times in total, significantly facilitated gastric pepsin secretion, although a single dose produced no significant effect.4 The PAR-2-mediated gastric pepsin secretion was resistant to omeprazole, N-G-nitro-L-arginine methyl ester (L-NAME) or atropine, and also to ablation of sensory neurons by capsaicin.5 Our study thus provides novel evidence that PAR-2 is localized in mucosal chief cells and facilitates gastric pepsin secretion in the rats. most probably by a direct mechanism.British Journal of Pharmacology (2002).