Method of isolation-perfusion of the liver in the pig.
Method of isolation-perfusion of the liver in the pig.
复制标题
猪肝脏的分离灌注方法。
作者：
Sindelar Wf
建立了猪肝分离灌注泵氧合器的方法，该方法在血管分离后通过门静脉输注血液，并在封闭循环系统中收集肝静脉流出物。门静脉和腔静脉的血流通过腔内导管或外部分流转移到肝脏周围。用放射性白蛋白示踪剂测量灌注回路向体循环的渗漏。10只猪在37℃或41℃下灌注30或60分钟，9只动物存活，肝酶短暂升高，但没有永久性肝损伤的临床或病理迹象。
: A method was developed in the pig for isolation-perfusion of the liver using a pump-oxygenator that infused blood through the portal vein after vascular isolation and collected the hepatic venous effluent in a closed recirculating system. Portal and vena caval blood flows were diverted around the liver by an intracaval conduit or by an external shunt. Leakage from the perfusion circuit into the systemic circulation was measured using radioactive albumin tracer. Ten pigs were perfused for 30 or 60 minutes at 37 C or 41 C. Nine animals survived treatment with transient elevations of hepatic enzymes but with no clinical or pathologic signs of permanent hepatic damage.