Microbubble Functionalization with Platelet Membrane Enables Targeting and Early Detection of Sepsis-Induced Acute Kidney Injury.
Microbubble Functionalization with Platelet Membrane Enables Targeting and Early Detection of Sepsis-Induced Acute Kidney Injury.
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血小板膜微泡功能化能够靶向和早期检测脓毒症引起的急性肾损伤。
DOI：
10.1002/adhm.202101628
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发表时间：
2021
影响因子：
10
通讯作者：
Yao Weifeng
中科院分区：
文献类型：
--
作者：
Yang Jing;Miao Xiaoyan;Guan Yu;Chen Chaojin;Chen Sufang;Zhang Xinmin;Xiao Xue;Zhang Zheng;Xia Zhengyuan;Yin Tinghui;Hei Ziqing;Yao Weifeng
脓毒症所致急性肾损伤(SAKI)的发病率和死亡率居高不下。分子超声早期发现可降低病死率，改善预后。受血小板与SAKI之间内在联系的启发，血小板膜包裹杂化微泡(Pla-MBS)是为早期识别SAKI而设计的。聚乳酸-微球是通过超声辅助脂质体与血小板复合而成，由从血小板中分离出来的固有的血小板膜组成。通过包被血小板膜，PLA-MB被赋予各种黏附受体(如整合素αIIbβ3)，为选择性黏附SAKI损伤的内皮细胞提供了有利条件。在大鼠SAKI模型中，通过结合分子超声成像和血小板膜的优势，Pla-MBS显示了类似于血小板的特性，并在常规的肾功能实验室标志物之前实现了对SAKI的早期靶向诊断。此外，肾脏中血小板结合蛋白(von Willebrand因子和纤维蛋白原)的表达与分子超声成像结果一致。总之，血小板膜的微泡功能化对SAKI的诊断是有益的，并且可能是未来治疗内皮损伤疾病的一种有前途的方式。
The morbidity and mortality of sepsis‐induced acute kidney injury (SAKI) remain high. Early detection using molecular ultrasound imaging may reduce mortality and improve the prognosis. Inspired by the intrinsic relationship between platelets and SAKI, platelet membrane‐coated hybrid microbubbles (Pla‐MBs) are designed for early recognition of SAKI. Pla‐MBs are prepared by ultrasound‐assisted recombination of liposomes and platelets, consisting of inherent platelet membrane isolated from platelets. By coating with platelet membranes, Pla‐MBs are endowed with various adhesive receptors (such as integrinαIIbβ3), providing a benefit for selective adhesion to damaged endothelium in SAKI. In a rat SAKI model, by combining the advantages of molecular ultrasound imaging and platelet membrane, Pla‐MBs display platelet‐mimicking properties and achieve the early targeted diagnosis of SAKI prior to the regular laboratory markers of kidney function. Moreover, the expression of platelet‐binding proteins (von Willebrand factor and fibrinogen) in the kidneys shows consistent results with molecular ultrasound imaging. Together, microbubble functionalization with platelet membranes is diagnostically beneficial for SAKI and might be a promising modality for endothelial injury diseases in the future.