Preparation of photothermal-chemotherapy nanohybrids by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains
Preparation of photothermal-chemotherapy nanohybrids by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains
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DOI：
10.1039/c9tb02163a
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发表时间：
2020-04-14
影响因子：
7
通讯作者：
Kono, Kenji
中科院分区：
文献类型：
--
作者：
Hashimoto, Takuya;Yuba, Eiji;Kono, Kenji
激光热疗与抗癌药物的联合应用，将是一种副作用小、疗效高的肿瘤治疗方法。本研究结合抗癌药物负载的功能性树枝状聚合物和光响应性金纳米棒来制造纳米杂化物，该纳米杂化物可以在近红外激光照射下提供抗癌药物递送和随后的产热。使用缩合反应将聚（乙二醇）改性的聚酰胺-胺树枝状聚合物缀合到羧基化的金纳米棒表面。将油酰基引入树枝状聚合物中以提高载药能力。通过将油酰基链并入纳米杂化物中的树枝状聚合物中来提高阿霉素负载能力，表明抗癌药物和纳米杂化物之间的疏水相互作用增加。在近红外激光照射下，纳米杂化材料表现出产热性能。它们会随着时间的推移释放抗癌药物。多柔比星装载的纳米杂化物和激光照射的组合显示出比使用激光的纳米杂化物和不使用激光的药物装载的纳米杂化物的细胞毒性明显更好。在将纳米杂交体静脉内或瘤内注射到荷瘤小鼠后，在激光照射下在肿瘤部位观察到急剧的温度升高。特别是，肿瘤内注射阿霉素负载的纳米杂化物在激光照射下显示出几乎完全的肿瘤生长抑制。结果表明，功能性树枝状聚合物-金纳米棒纳米杂化物作为多功能纳米材料是有希望的，以实现抗癌药物和热消融的协同效应，以支持有效的癌症治疗。
The combination of anticancer drugs and laser hyperthermia could lead to efficient cancer treatment with less-adverse effects. This study combined anticancer drug-loaded functional dendrimers and light-responsive gold nanorods to fabricate nanohybrids that can provide anticancer-drug delivery and subsequent heat generation under near-infrared laser irradiation. A condensation reaction was used to conjugate poly(ethylene glycol)-modified polyamidoamine dendrimers to carboxylated gold nanorod surfaces. Oleoyl groups were incorporated into dendrimers to improve the drug loading capacity. Doxorubicin loading capacity was improved by incorporation of oleoyl chains into dendrimers in the nanohybrid, indicating increased hydrophobic interaction between anticancer drugs and nanohybrids. The nanohybrids exhibited heat generation properties under near infrared laser irradiation. They released anticancer drugs over time. The combination of doxorubicin-loaded nanohybrids and laser irradiation showed markedly better cytotoxicity than that of the nanohybrids used with lasers and drug-loaded nanohybrids without the use of lasers. After intravenous or intratumoral injection of nanohybrids to tumor-bearing mice, a sharp temperature increase was observed at the tumor site under laser irradiation. Especially, intratumorally injected doxorubicin-loaded nanohybrids showed almost complete tumor growth suppression under laser irradiation. The results demonstrate that functional dendrimer-gold nanorod nanohybrids are promising as multi-functional nanomaterials to achieve synergistic effects of anticancer drugs and heat ablation to support effective cancer treatments.