Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease
Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease
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DOI：
10.1094/phyto-02-21-0054-sc
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发表时间：
2021-12-01
期刊：
影响因子：
3.2
通讯作者：
Vetukuri, Ramesh R.
中科院分区：
文献类型：
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作者：
Kalyandurg, Pruthvi B.;Sundararajan, Poorva;Vetukuri, Ramesh R.
致病疫霉在马铃薯和番茄上引起晚疫病，目前通过抗性品种或密集的杀真菌剂喷洒来控制。在这里，我们研究了一种控制晚疫病的替代方法，即用靶向致病疫霉感染所必需的基因的双链RNA（dsRNA）喷洒马铃薯叶片。首先，我们发现表达绿色荧光蛋白（GFP）的致病疫霉孢子囊可以直接从其周围环境（包括叶片）摄取体外合成的与GFP同源的dsRNA，这导致GFP的相对表达降低。我们进一步证明了喷雾诱导基因沉默（SIGS）在控制马铃薯晚疫病中的潜力，其通过靶向致病疫霉中的发育重要基因，如鸟嘌呤核苷酸结合蛋白β亚基（PiGPB 1）、吸器膜蛋白（PiHmp 1）、角质酶（PiCut 3）和内切-1，3（4）-β-葡聚糖酶（PiEndo 3）。我们的研究结果表明，SIGS可以潜在地用于减轻马铃薯晚疫病;然而，疾病控制的程度取决于靶基因的选择。
Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein beta-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-beta-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.