Protein adsorption on biodegradable polyanhydride microparticles
Protein adsorption on biodegradable polyanhydride microparticles
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DOI：
10.1002/jbm.a.32815
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发表时间：
2010-10-01
影响因子：
4.9
通讯作者：
Narasimhan, Balaji
中科院分区：
文献类型：
--
作者：
Carrillo-Conde, Brenda;Garza, Alicia;Narasimhan, Balaji
研究了以癸二酸（SA）、1，6-二（对羧基苯氧基）己烷（CPH）和1，8-二（对羧基苯氧基）-3，6-二氧杂辛烷（CPTEG）为主要成分的聚酸酐微粒对血浆蛋白的体外吸附。使用来自牛血清的三种模型蛋白（白蛋白（BSA）、免疫球蛋白G（IgG）和纤维蛋白原（Fg））。采用X射线光电子能谱和凝胶电泳研究了吸附。应用二维电泳技术研究了牛血清中血浆蛋白的吸附。蛋白质吸附量的差异被检测为以下函数：（i）共聚物组成和（ii）研究的特定蛋白质。观察到聚合物疏水性和蛋白质吸附之间的直接相关性，并且吸收了更大量的Fg和IgG。用与Fg孵育的卵清蛋白包封的微粒进行体外释放研究;这些研究表明，当Fg吸附在表面上时，从微粒释放的卵清蛋白的量减少。了解胃肠外给药装置上的蛋白质吸附模式对于优化其体内性能是有价值的。(C)2010 Wiley Periodicals，Inc. J Biomed Mater Res Part A：95A：40-48，2010.
The in vitro adsorption of plasma proteins on polyanhydride microparticles based on sebacic acid (SA), 1,6-bis(p-carboxyphenoxy)hexane (CPH), and 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) was studied. Three model proteins from bovine serum (albumin (BSA), immunoglobulin G (IgG), and fibrinogen (Fg)) were used. The adsorption was studied using X-Ray Photoelectron Spectroscopy and gel electrophoresis. 2D electrophoresis was used to study the adsorption of plasma proteins from bovine serum. Differences in the amount of protein adsorbed were detected as a function of the following: (i) copolymer composition and (ii) specific protein studied. A direct correlation between polymer hydrophobicity and protein adsorbed was observed and higher quantities of Fg and IgG were absorbed. In vitro release studies were performed with ovalbumin-encapsulated microparticles that were incubated with Fg; these studies showed a reduction in the amount of ovalbumin released from the microparticles when Fg is adsorbed on the surface. An understanding of protein adsorption patterns on parenteral delivery devices is valuable in optimizing their in vivo performance. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 40-48, 2010.