Arabidopsis plants lacking PsbQ and PsbR subunits of the oxygen-evolving complex show altered PSII super-complex organization and short-term adaptive mechanisms
Arabidopsis plants lacking PsbQ and PsbR subunits of the oxygen-evolving complex show altered PSII super-complex organization and short-term adaptive mechanisms
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DOI：
10.1111/tpj.12230
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发表时间：
2013-08-01
期刊：
影响因子：
7.2
通讯作者：
Pesaresi, Paolo
中科院分区：
文献类型：
--
作者：
Allahverdiyeva, Yagut;Suorsa, Marjaana;Pesaresi, Paolo
真核生物光系统II（PSII）的放氧复合体由PsbO（33 kDa）、PsbP（23 kDa）、PsbQ（17 kDa）和PsbR（10 kDa）4个外源亚基组成，由7个核基因PsbO 1编码，（At 5g 66570）、PsbO 2（At 3g 50820）、PsbP 1（At 1g 06680）、PsbP 2（At 2g 30790）、PsbQ 1（At 4g 21280）、PsbQ 2（At 4g 05180）和PsbR（At 1g 79040）。使用拟南芥插入突变株系，我们表明，PsbP 1，但不是PsbP 2，是必不可少的光合自养生长，而植物缺乏这两种形式的PsbQ和/或PsbR显示正常的增长率。完全消除PsbQ对PSII功能的影响较小，但缺乏PsbR或PsbR和PsbQ的植物的特征在于PSII活性的更明显的缺陷。基因表达和免疫印迹分析表明，这些蛋白质中的每一个的积累是高度依赖于其他的存在下，并在转录后水平控制，而PsbO稳定性似乎是不太敏感的耗氧复合物的其他亚基。此外，在野生型和突变体叶片中的PSII超复合物的水平的比较揭示了氧进化复合物的超分子组织的PSII和它们的影响的状态转换的速率的各个亚基的重要性。
The oxygen-evolving complex of eukaryotic photosystemII (PSII) consists of four extrinsic subunits, PsbO (33kDa), PsbP (23kDa), PsbQ (17kDa) and PsbR (10kDa), encoded by seven nuclear genes, PsbO1 (At5g66570), PsbO2 (At3g50820), PsbP1 (At1g06680), PsbP2 (At2g30790), PsbQ1 (At4g21280), PsbQ2 (At4g05180) and PsbR (At1g79040). Using Arabidopsis insertion mutant lines, we show that PsbP1, but not PsbP2, is essential for photoautotrophic growth, whereas plants lacking both forms of PsbQ and/or PsbR show normal growth rates. Complete elimination of PsbQ has a minor effect on PSII function, but plants lacking PsbR or both PsbR and PsbQ are characterized by more pronounced defects in PSII activity. Gene expression and immunoblot analyses indicate that accumulation of each of these proteins is highly dependent on the presence of the others, and is controlled at the post-transcriptional level, whereas PsbO stability appears to be less sensitive to depletion of other subunits of the oxygen-evolving complex. In addition, comparison of levels of the PSII super-complex in wild-type and mutant leaves reveals the importance of the individual subunits of the oxygen-evolving complex for the supramolecular organization of PSII and their influence on the rate of state transitions.