Spectral Sensitivity Measured with Electroretinogram Using a Constant Response Method.
Spectral Sensitivity Measured with Electroretinogram Using a Constant Response Method.
复制标题
DOI：
10.1371/journal.pone.0147318
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ventura DF
中科院分区：
文献类型：
--
作者：
Rocha FA;Gomes BD;Silveira LC;Martins SL;Aguiar RG;de Souza JM;Ventura DF
提出了一种利用视网膜电图（ERG）测定视网膜光谱敏感度函数S（λ）的新方法。在三种不同种属的肌型啮齿动物（长爪沙鼠、Wistar大鼠和小鼠）中评估S（λ）。该方法被称为AC恒定方法，基于计算机化的自动反馈系统，该系统调节光强度以在整个光谱中保持对闪烁刺激的恒定响应幅度，因为它从300 nm扫描到700 nm，然后返回。结果表示为保持恒定峰间响应幅度所需的每个波长处的强度的倒数。由此产生的S（λ）在所有三种啮齿类动物中都有两个峰，分别对应于紫外和M视锥：小鼠为359 nm和511 nm，沙鼠为362 nm和493 nm，大鼠为362 nm和502 nm。小鼠和沙鼠的结果与其他方法获得的S（λ）函数的文献报道相似，证实了与AC恒定反应方法相关的ERG可有效获得可靠的S（λ）函数。此外，由于其快速的数据收集时间，AC恒定响应方法具有保持眼睛处于恒定光适应状态的优点。
A new method is presented to determine the retinal spectral sensitivity function S(λ) using the electroretinogram (ERG). S(λ)s were assessed in three different species of myomorph rodents, Gerbils (Meriones unguiculatus), Wistar rats (Ratus norvegicus), and mice (Mus musculus). The method, called AC Constant Method, is based on a computerized automatic feedback system that adjusts light intensity to maintain a constant-response amplitude to a flickering stimulus throughout the spectrum, as it is scanned from 300 to 700 nm, and back. The results are presented as the reciprocal of the intensity at each wavelength required to maintain a constant peak to peak response amplitude. The resulting S(λ) had two peaks in all three rodent species, corresponding to ultraviolet and M cones, respectively: 359 nm and 511 nm for mice, 362 nm and 493 nm for gerbils, and 362 nm and 502 nm for rats. Results for mouse and gerbil were similar to literature reports of S(λ) functions obtained with other methods, confirming that the ERG associated to the AC Constant-Response Method was effective to obtain reliable S(λ) functions. In addition, due to its fast data collection time, the AC Constant Response Method has the advantage of keeping the eye in a constant light adapted state.